CAJ | 학술논문

目的：探讨益气化瘀方促进产后子宫复旧的作用机制。方法选取50只雌性SD大鼠,采用阴道内注射大肠杆菌制备产后子宫复旧不全模型。按妊娠先后次序编号,随机分为空白组、模型组、新生化组及益气化瘀方低、高剂量组,空白组和模型组灌胃纯净水,益气化瘀方低、高剂量组给予益气化瘀方灌胃,新生化组给予新生化颗粒灌胃,每日1次,连续7 d。HE 染色观察子宫内膜形态,硝酸还原酶法检测大鼠血清中一氧化氮(NO)、一氧化氮合酶(NOS)的含量。结果模型组大鼠子宫组织切片HE染色示黏膜下层充血、出血,内膜连续性中断,伴炎性细胞浸润,提示造模成功。模型组NO、NOS含量均高于空白组；与模型组比较,各给药组NO、NOS明显降低(P＜0.05,P＜0.01)；与新生化组比较,益气化瘀高剂量组NO、NOS降低(P＜0.05)。结论益气化瘀方通过降低NO、NOS水平,抑制子宫螺旋动脉异常舒张,促进子宫内膜的重建与修复。
목적：탐토익기화어방촉진산후자궁복구적작용궤제。방법선취50지자성SD대서,채용음도내주사대장간균제비산후자궁복구불전모형。안임신선후차서편호,수궤분위공백조、모형조、신생화조급익기화어방저、고제량조,공백조화모형조관위순정수,익기화어방저、고제량조급여익기화어방관위,신생화조급여신생화과립관위,매일1차,련속7 d。HE 염색관찰자궁내막형태,초산환원매법검측대서혈청중일양화담(NO)、일양화담합매(NOS)적함량。결과모형조대서자궁조직절편HE염색시점막하층충혈、출혈,내막련속성중단,반염성세포침윤,제시조모성공。모형조NO、NOS함량균고우공백조；여모형조비교,각급약조NO、NOS명현강저(P＜0.05,P＜0.01)；여신생화조비교,익기화어고제량조NO、NOS강저(P＜0.05)。결론익기화어방통과강저NO、NOS수평,억제자궁라선동맥이상서장,촉진자궁내막적중건여수복。
Objective To explore the action mechanism of Yiqi Huayu Formula for postpartum subinvolution of uterus. Methods Fifty female SD rats were selected model with postpartum subinvolution of uterus was made by injecting escherichia coli in vagina. Rats were randomly divided into 5 groups according to pregnancy time:blank group, model group, Xinshenghua group, and Yiqi Huayu high and low dose groups. Blank group and model group received gavage with water;Yiqi Huayu low and high dose groups received gavage with Yiqi Huayu Formula;Xinshenghua group received gavage with Xinshenghua Granule, once a day for 7 days. Uterine endometrial morphology was observed by HE staining. The contents of NO and NOS were respectively detected by Nitrate reductase method. Results Uterus submucosal hyperemia, bleeding, and endometrial discontinuity with the infiltration of inflammatory cells of rats in the model group suggested that the models were successfully established. Compared with blank group, the contents of NO and NOS in model group were higher significantly. Compared with model group, the contents of NO and NOS significantly decreased in both Xinshenghua group and Yiqi Huayu high and low groups (P<0.01, P<0.01). Compared with Xinshenghua group, Yiqi Huayu high dose group was lower (P<0.05). Conclusion Yiqi Huayu Formula could promote reconstruction and repair of the endometrium by reducing the contents of NO and NOS and inhibiting abnormal uterine spiral arteries relaxation.