CAJ | 학술논문

目的：观察子午流注纳甲法对急性缺血性脑卒中(AIS)模型大鼠血清神经元特异性烯醇化酶(NSE)、S100B蛋白含量的影响。方法选用SPF级SD雄性大鼠,采用大脑中动脉线栓法建立模型,按随机数字表法分为空白组、假手术组、模型组、循经组和纳甲组,空白组、假手术组和模型组不作治疗,循经组予循经辨证取穴针刺治疗,纳甲组予子午流注纳甲法针刺治疗,每日定时治疗1次,观察神经功能及脑梗死体积情况,检测血清NSE、S100B蛋白的含量。结果与模型组比较,循经组、纳甲组神经功能评分下降、梗死灶体积减小、梗死灶体积百分比降低(P＜0.05,P＜0.01),血清 NSE、S100B 蛋白含量降低(P＜0.05,P＜0.01),纳甲组效果总体优于循经组。结论子午流注纳甲法通过降低AIS模型大鼠血清NSE、S100B蛋白含量达到神经保护和治疗作用。
목적：관찰자오류주납갑법대급성결혈성뇌졸중(AIS)모형대서혈청신경원특이성희순화매(NSE)、S100B단백함량적영향。방법선용SPF급SD웅성대서,채용대뇌중동맥선전법건립모형,안수궤수자표법분위공백조、가수술조、모형조、순경조화납갑조,공백조、가수술조화모형조불작치료,순경조여순경변증취혈침자치료,납갑조여자오류주납갑법침자치료,매일정시치료1차,관찰신경공능급뇌경사체적정황,검측혈청NSE、S100B단백적함량。결과여모형조비교,순경조、납갑조신경공능평분하강、경사조체적감소、경사조체적백분비강저(P＜0.05,P＜0.01),혈청 NSE、S100B 단백함량강저(P＜0.05,P＜0.01),납갑조효과총체우우순경조。결론자오류주납갑법통과강저AIS모형대서혈청NSE、S100B단백함량체도신경보호화치료작용。
Objective To observe the effects of Najia method of midday-midnight point selection for acute ischemic stroke (AIS) model rats onthe contents of NSE and S100B protein in serum. Methods SPF SD male rats were chosen to establish the models by middle cerebral artery bolt method. Rats were divided into blank group, sham-operation group, model group, channel-point group, and Najia method group by random number table method. Blank group, sham-operation group, and model group were in the absence of treatment, while the channel-point group received acupuncture treatment according to differentiation syndrome. Najia method group used Najia method of midday-midnight point selection to conduct acupuncture treatment once a day. Improvement of neural function and cerebral infarction volume were observed. The contents of NSE and S100B protein in serum were detected. Results Compared with model group, neurological function score, infarct volume and infarct volume percentage, and the contents of NSE and S100B protein in serum decreased in Najia method group and channel-point group (P<0.05, P<0.01). The effects of Najia group were generally better than the channel-point group. Conclusion Najia method of midday-midnight point selection can decrease the content of NSE and S100B protein in serum of AIS model rats, so as to achieve the effects of neuroprotection and treatment.