CAJ | 학술논문

目的：探讨磷脂酰肌醇3激酶抑制剂 LY294002对人胚胎干细胞定向分化为成熟胰岛素分泌细胞的影响。方法：体外通过5个阶段诱导人胚胎干细胞定向分化为胰岛素分泌细胞。分别给予尼克酰胺＋B27（B27组）为对照组和尼克酰胺＋ LY294002（LY组）为实验组诱导胰岛素分泌细胞的成熟。显微镜下观察各阶段细胞形态变化，免疫荧光染色鉴定胰岛素、c－肽、生长抑素和胰高血糖素的表达。结果：第5阶段诱导14 d LY组胰岛素单染阳性率与B27组无统计学差别（P﹥0．05），但生长抑素和胰高血糖素单染阳性率，胰岛素／生长抑素共染率均低于B27组（P﹤0．05）。结论：在无血清培养体系下，磷脂酰肌醇3激酶抑制剂LY294002能够诱导人胚胎干细胞分化为更加成熟的胰岛素分泌细胞。
목적：탐토린지선기순3격매억제제 LY294002대인배태간세포정향분화위성숙이도소분비세포적영향。방법：체외통과5개계단유도인배태간세포정향분화위이도소분비세포。분별급여니극선알＋B27（B27조）위대조조화니극선알＋ LY294002（LY조）위실험조유도이도소분비세포적성숙。현미경하관찰각계단세포형태변화，면역형광염색감정이도소、c－태、생장억소화이고혈당소적표체。결과：제5계단유도14 d LY조이도소단염양성솔여B27조무통계학차별（P﹥0．05），단생장억소화이고혈당소단염양성솔，이도소／생장억소공염솔균저우B27조（P﹤0．05）。결론：재무혈청배양체계하，린지선기순3격매억제제LY294002능구유도인배태간세포분화위경가성숙적이도소분비세포。
Objective To investigate the effect of phosphoinositide-3-kinase inhibitor LY294002 on the differentiation of human embryonic stem cells (HESC) into more mature insulin-producing cells. Methods HESCs were induced to differentiate into insulin-producing cells through five stages. Nicotinamide and B27 (group B27), nicotinamide and LY294002 (group LY) were used to induce the nesting positive cells into mature insulin-producing cells. The morphological change of each stage was observed under microscope , and expressions of insulin, c-peptide, somatostatin and glucagon were identified by immunofluorescence staining. Results After 14 days in stage 5 , there was no significant difference in rate of insulin positive cells between group LY and group B27 (P﹥0.05), but rates of somatostatin and glucagon positive cells in group LY were lower than those in group B27(P﹤0.05). Furthermore, the co-stained rate of somatostatin and insulin in group LY was also lower than that in group B27 (P﹤0.05). Conclusion HESCs can be induced to differentiate into more mature insulin-producing cells by phosphoinositide-3-kinase inhibitor LY294002 in serum-free culture medium.