CAJ | 학술논문

目的：观察Mus81基因沉默对人结肠癌细胞HCT116奥沙利铂敏感性的影响并分析其机制。方法：采用慢病毒介导的 RNAi 技术，构建 Mus81基因沉默的 HCT116细胞，MTT 法检测奥沙利铂的 IC50值。Annexin V-APC 染色法检测奥沙利铂处理后的细胞凋亡率，并以实时定量 PCR 及 Western blot 检测 p53、Bax及Bcl-2的表达水平。结果：Mus81沉默的HCT116细胞对奥沙利铂的IC50值明显降低，该细胞经奥沙利铂处理后凋亡率上升了77．6％（P ＜0．01），p53和Bax 的表达明显升高（P ＜0．01），Bcl-2表达则明显降低（P ＜0．01），导入Mus81基因则可降低该细胞凋亡率。结论：Mus81基因沉默可提高HCT116细胞对奥沙利铂的敏感性，其机制可能是调控p53、Bax及Bcl-2的表达从而促进细胞凋亡。
목적：관찰Mus81기인침묵대인결장암세포HCT116오사리박민감성적영향병분석기궤제。방법：채용만병독개도적 RNAi 기술，구건 Mus81기인침묵적 HCT116세포，MTT 법검측오사리박적 IC50치。Annexin V-APC 염색법검측오사리박처리후적세포조망솔，병이실시정량 PCR 급 Western blot 검측 p53、Bax급Bcl-2적표체수평。결과：Mus81침묵적HCT116세포대오사리박적IC50치명현강저，해세포경오사리박처리후조망솔상승료77．6％（P ＜0．01），p53화Bax 적표체명현승고（P ＜0．01），Bcl-2표체칙명현강저（P ＜0．01），도입Mus81기인칙가강저해세포조망솔。결론：Mus81기인침묵가제고HCT116세포대오사리박적민감성，기궤제가능시조공p53、Bax급Bcl-2적표체종이촉진세포조망。
Objective To investigate the effects of Mus81 gene silencing on the chemosensitivity of human colon cancer HCT116 cells to oxaliplatin and explore the underlying mechanisms. Methods lentiviral-mediated RNAi was employed to inhibit Mus81 gene in human colon cancer HCT116 cells. MTT assay was employed to evaluate oxaliplatin chemosensitivity of HCT116. Cell apoptosis rate was detected by Annexin V-APC staining and the expression levels of p53, while Bax and Bcl-2 in HCT116 cells were detected by Real-time PCR and Western blot. Results Oxaliplatin IC50 of Mus81 depleted HCT116 cells was significantly decreased. After oxaliplatin treatment, the cell apoptosis rate of Mus81 silenced HCT116 cells was increased by 77.6% (P < 0.05), and the expression of p53 and Bax in these cells was obviously increased but the expression of Bcl-2 was significantly decreased (P < 0.01). Introducing Mus81 gene in Mus81 depleted HCT116 cells could reduce apoptosis rate. Conclusion Mus81 gene silencing can significantly improve oxliplatin chemosensitivity of HCT116 cells possibly by promoting cell apoptosis through regulating the expression of p53, Bax and Bcl-2.