CAJ | 학술논문

采用亨盖特（Hungate）厌氧滚管技术从锦州湾受砷污染的底泥中分离纯化出3株具有砷（V）还原活性的硫酸盐还原菌，分别编号为S2、S3-11和S13.16S rRNA基因序列分析显示这3株菌分别与梭菌属（Clostridium）内的不同“种”之间亲缘关系最近.在As（V）初始浓度为1.0 mmol·L-1时，菌株S3-11可在10 h内还原23.4%的As（V），但当As（V）初始浓度增加到3.0或5.0 mmol·L-1时，菌株S2和S13则相对于S3-11展现出更强的砷还原能力，菌株S2甚至可以在7.0 mmol·L-1的砷环境中生长并在24 h内将14.2%的As（V）还原.菌株S2为严格厌氧菌，为直杆状革兰氏阳性菌，产芽孢，其细胞大小约为2.0μm×0.6μm，16S rRNA基因序列与梭菌属中Clostridium sporogenes strain JCM 7849同源性为99%.菌株S2可利用蔗糖、葡萄糖、甲酸钠、乳酸钠和乙酸钠为唯一碳源生长，生长适宜温度为30℃.
채용형개특（Hungate）염양곤관기술종금주만수신오염적저니중분리순화출3주구유신（V）환원활성적류산염환원균，분별편호위S2、S3-11화S13.16S rRNA기인서렬분석현시저3주균분별여사균속（Clostridium）내적불동“충”지간친연관계최근.재As（V）초시농도위1.0 mmol·L-1시，균주S3-11가재10 h내환원23.4%적As（V），단당As（V）초시농도증가도3.0혹5.0 mmol·L-1시，균주S2화S13칙상대우S3-11전현출경강적신환원능력，균주S2심지가이재7.0 mmol·L-1적신배경중생장병재24 h내장14.2%적As（V）환원.균주S2위엄격염양균，위직간상혁란씨양성균，산아포，기세포대소약위2.0μm×0.6μm，16S rRNA기인서렬여사균속중Clostridium sporogenes strain JCM 7849동원성위99%.균주S2가이용자당、포도당、갑산납、유산납화을산납위유일탄원생장，생장괄의온도위30℃.
Three sulfate-reducing bacterial strains that can reduce arsenate to arsenite,designated as S2,S3-11, and S13,were isolated by Hungate anaerobic techniques from arsenic-contaminated estuarial sediment at Jinzhou bay , China. Analysis of 16S rRNA gene sequences suggest that these strains are most similar to species from Clostridium. In presence of 1.0 mmol·L-1 As(V),strain S3-11 could reduce 23.4%of arsenate within 10 hours;however,strain S2 and Strain S13 showed relatively higher arsenate reduction efficacy compared to strain S3-11 when 3.0 mmol·L-1 or 5.0 mmol·L-1 arsenate was present. Strain S2 could grow with 7.0 mmol·L-1 and reduced 14.2%of arsenate in a 24 h incubation. Strain S2 was a rod-shape,Gram-positive,spore-forming,and strictly anaerobic bacterium. The cells of S2 were 2.0×0.6μm in size. Strain S2 shares a 99%sequence similarity in 16S rRNA gene with Clostridium sporogens strain JCM 7849. Sucrose,glucose,sodium formate,sodium acetate,and sodium lactate could be used as the sole carbon source by strain S2 and the optimum temperature for growth was 30℃.