医学研究与教育
醫學研究與教育
의학연구여교육
MEDICAL RESEARCH AND EDUCATION
2015年
2期
11-15,34
,共6页
黄独乙素%胃肿瘤%SGC-7901细胞株%增殖%CyclinD1蛋白
黃獨乙素%胃腫瘤%SGC-7901細胞株%增殖%CyclinD1蛋白
황독을소%위종류%SGC-7901세포주%증식%CyclinD1단백
Disobulbin B%gastric carcinoma%SGC-7901 cells%proliferation%CyclinD1 protein
目的：研究黄独乙素对人胃癌细胞 SGC-7901增殖的影响及相关的作用机制。方法用RPMI-1640培养液对SGC-7901细胞进行传代培养，用不同浓度（0~160μg/mL）黄独乙素分别对SGC-7901细胞进行处理，应用CCK-8法检测细胞的增殖率；光学显微镜观察细胞形态学改变；Western blot检测细胞周期蛋白CyclinD1表达的变化。结果黄独乙素以计量依赖方法抑制胃癌细胞生长，抑制率最高达57.26％；细胞形态发生明显改变；CyclinD1蛋白水平表达显著下调。结论黄独乙素能显著抑制SGC-7901细胞的增殖，其机制可能与下调细胞周期蛋白CyclinD1的表达有关。
目的：研究黃獨乙素對人胃癌細胞 SGC-7901增殖的影響及相關的作用機製。方法用RPMI-1640培養液對SGC-7901細胞進行傳代培養，用不同濃度（0~160μg/mL）黃獨乙素分彆對SGC-7901細胞進行處理，應用CCK-8法檢測細胞的增殖率；光學顯微鏡觀察細胞形態學改變；Western blot檢測細胞週期蛋白CyclinD1錶達的變化。結果黃獨乙素以計量依賴方法抑製胃癌細胞生長，抑製率最高達57.26％；細胞形態髮生明顯改變；CyclinD1蛋白水平錶達顯著下調。結論黃獨乙素能顯著抑製SGC-7901細胞的增殖，其機製可能與下調細胞週期蛋白CyclinD1的錶達有關。
목적：연구황독을소대인위암세포 SGC-7901증식적영향급상관적작용궤제。방법용RPMI-1640배양액대SGC-7901세포진행전대배양，용불동농도（0~160μg/mL）황독을소분별대SGC-7901세포진행처리，응용CCK-8법검측세포적증식솔；광학현미경관찰세포형태학개변；Western blot검측세포주기단백CyclinD1표체적변화。결과황독을소이계량의뢰방법억제위암세포생장，억제솔최고체57.26％；세포형태발생명현개변；CyclinD1단백수평표체현저하조。결론황독을소능현저억제SGC-7901세포적증식，기궤제가능여하조세포주기단백CyclinD1적표체유관。
Objective To investigate effects of Disobulbin B (DB) on the proliferation of human gastric carcinoma cell line SGC-7901 and explore the possible mechanism. Methods SGC-7901 cells were treated with different concentration (0~160μg/mL) of DB. Cell proliferation was detected by CCK-8 assay;cell morphology changes were observed under optical microscopy;The expression of CyclinD1 was detected by Western blot. Results DB inhibited the growth of gastric cancer cells in a does-dependent manner and the highest inhibition rate was 57.26%.The structure of SGC-7901 cells changed greatly. The protein expression level of cyclinD1 was reduced. The effects were increased with the increase of the DB concentration. Conclusion DB could inhibit cell proliferation in SGC-7901 cells by down-regulating the expression of CyclinD1.
