医学研究与教育
醫學研究與教育
의학연구여교육
MEDICAL RESEARCH AND EDUCATION
2015年
2期
1-5
,共5页
乔亚君%高颖%兰丹丹%梁洁%李建恒
喬亞君%高穎%蘭丹丹%樑潔%李建恆
교아군%고영%란단단%량길%리건항
AM真菌%丹参%多糖%硒%丹参酮ⅡA
AM真菌%丹參%多糖%硒%丹參酮ⅡA
AM진균%단삼%다당%서%단삼동ⅡA
AM fungi%Salvia miltiorrhiza Bge%polysaccharide%Selenium%tanshinone IIA
目的：研究不同施硒水平下接种AM真菌对丹参幼苗品质的影响。方法利用盆栽试验得到不同生长条件下丹参幼苗，通过测定多糖、硒、丹参酮ⅡA含量评价丹参品质。结果同一施硒水平下，接种AM真菌显著提高丹参幼苗多糖、微量元素硒的质量分数，表明AM真菌能够促进丹参对营养物质的积累，增强其对微量元素的吸收。且接种株丹参酮ⅡA质量分数均高于未接种株，当硒质量浓度为40μg/mL时接种株丹参酮ⅡA质量分数最高，为0.272%；不同施硒水平下，随硒质量浓度的增加，丹参多糖与丹参酮ⅡA 含量均先增大后减小，但接种株含量高于未接种株，在Se1（20μg/mL）~Se2（40μg/mL）时达到最高，说明高硒反而抑制药用成分的富集。由此看出适量质量浓度的硒与AM真菌具有协同作用，能够更有效地促进丹参营养和药用成分的积累。丹参中硒质量分数与施硒质量浓度呈显著正相关，但无明显线性关系，且硒的分布为茎叶＞根部。结论硒质量浓度为20~40μg/mL时AM真菌对丹参的接种效果最佳。
目的：研究不同施硒水平下接種AM真菌對丹參幼苗品質的影響。方法利用盆栽試驗得到不同生長條件下丹參幼苗，通過測定多糖、硒、丹參酮ⅡA含量評價丹參品質。結果同一施硒水平下，接種AM真菌顯著提高丹參幼苗多糖、微量元素硒的質量分數，錶明AM真菌能夠促進丹參對營養物質的積纍，增彊其對微量元素的吸收。且接種株丹參酮ⅡA質量分數均高于未接種株，噹硒質量濃度為40μg/mL時接種株丹參酮ⅡA質量分數最高，為0.272%；不同施硒水平下，隨硒質量濃度的增加，丹參多糖與丹參酮ⅡA 含量均先增大後減小，但接種株含量高于未接種株，在Se1（20μg/mL）~Se2（40μg/mL）時達到最高，說明高硒反而抑製藥用成分的富集。由此看齣適量質量濃度的硒與AM真菌具有協同作用，能夠更有效地促進丹參營養和藥用成分的積纍。丹參中硒質量分數與施硒質量濃度呈顯著正相關，但無明顯線性關繫，且硒的分佈為莖葉＞根部。結論硒質量濃度為20~40μg/mL時AM真菌對丹參的接種效果最佳。
목적：연구불동시서수평하접충AM진균대단삼유묘품질적영향。방법이용분재시험득도불동생장조건하단삼유묘，통과측정다당、서、단삼동ⅡA함량평개단삼품질。결과동일시서수평하，접충AM진균현저제고단삼유묘다당、미량원소서적질량분수，표명AM진균능구촉진단삼대영양물질적적루，증강기대미량원소적흡수。차접충주단삼동ⅡA질량분수균고우미접충주，당서질량농도위40μg/mL시접충주단삼동ⅡA질량분수최고，위0.272%；불동시서수평하，수서질량농도적증가，단삼다당여단삼동ⅡA 함량균선증대후감소，단접충주함량고우미접충주，재Se1（20μg/mL）~Se2（40μg/mL）시체도최고，설명고서반이억제약용성분적부집。유차간출괄량질량농도적서여AM진균구유협동작용，능구경유효지촉진단삼영양화약용성분적적루。단삼중서질량분수여시서질량농도정현저정상관，단무명현선성관계，차서적분포위경협＞근부。결론서질량농도위20~40μg/mL시AM진균대단삼적접충효과최가。
Objective To explore the effects of AM fungi on the quality of Salvia miltiorrhiza under different selenium levels. Methods Salvia miltiorrhiza seedlings under different conditions were obtained by pot experiment, and the content of polysaccharide, selenium and tanshinone IIA were determinated to evaluate the quality of plants. Results Inoculation of AM fungi signiifcantly increased polysaccharide and selenium content of Salvia miltiorrhiza under the same selenium level. It can be seen AM fungi can promote the accumulation of nutrients, improve the uptake of nutrient elements. The contents of tanshinone IIA in inoculated plants were higher than non inoculated plants. While the selenium concentration was 40μg/mL, the content of tanshinone IIA in inoculated plants was highest, that was 0.272%. On the different selenium levels, the content of polysaccharide and tanshinone IIA increased ifrstly and then decreased with increasing selenium concentration, but the contents of inoculated plants were higher than non inoculated plants, they reached the maximum at Se1(20μg/mL)-Se2(40μg/mL), showed that high selenium inhibited the accumulation of medicinal components. From that proper concentration of selenium was cooperated with AM fungi on improving the accumulating of polysaccharide and medicinal components of Salvia miltiorrhiza. Selenium content in Salvia miltiorrhiza Bge was positively correlated with selenium concentrations, but no obvious linear relationship, the distribution was more in the leaf than root. Conclusion Inoculation of AM fungi on the Salvia miltiorrhiza had the best effects when the selenium concentration was 20-40μg/mL.
