CAJ | 학술논문

根据香菇单核菌丝体135A 和135B 的全基因组测序，通过生物信息学方法分析筛选出香菇功能基因 CAP、GLA1、TLG1结构域中的非同义 SNP 位点，开发出3对 SNP-CAPS 分子标记。结果表明：这3对功能基因SNP-CAPS 分子标记可将23个香菇单核体供试菌株分成135A 型（分别为16株、20株、2株）和135B 型（分别为7株、3株、21株），这些标记表现出的多态性为进一步研究香菇菌株的来源和功能基因的定位提供参考价值；并建立起可以简单、快速、准确地调查重要功能基因的 SNP 位点变异情况的方法。
근거향고단핵균사체135A 화135B 적전기인조측서，통과생물신식학방법분석사선출향고공능기인 CAP、GLA1、TLG1결구역중적비동의 SNP 위점，개발출3대 SNP-CAPS 분자표기。결과표명：저3대공능기인SNP-CAPS 분자표기가장23개향고단핵체공시균주분성135A 형（분별위16주、20주、2주）화135B 형（분별위7주、3주、21주），저사표기표현출적다태성위진일보연구향고균주적래원화공능기인적정위제공삼고개치；병건립기가이간단、쾌속、준학지조사중요공능기인적 SNP 위점변이정황적방법。
Based on the whole genome sequencing of monokaryon strain of Lentinula edodes 135A and 135B,the non-synonymous SNP loci within the domain of the CAP,GLA1,TLG1 genes were found by using the bioinformatics way,and developed into three pairs of primers as SNP-CAPS molecular markers.The results showed that these SNP-CAPS markers could divide 23 tested monokaryons strains of L.edodes into two groups:135A type(corresponding 16,20 and 2 strains)and 135B type(corresponding 7,3 and 21 strains)which estab-lished the method to detect the distribution of known SNP loci in the population of L.edodes.This method will be helpful for tracking the source of L.edodes strains and identifying the functional geneseasily,quickly,and accu-rately.