CAJ | 학술논문

目的：优选裸花紫珠总黄酮的大孔吸附树脂纯化工艺。方法以总黄酮的吸附率和洗脱率为指标,采用静态吸附优选AB-8、D-101、HP-20、HP2MG等大孔吸附树脂的型号；通过单因素实验优选裸花紫珠的动态吸附分离参数。结果 HP-20型树脂的吸附效率较高,其最佳的纯化工艺为 pH 3.0的4.46 mg · mL-1裸花紫珠提取液,以3 BV·h-1流速上样,3 BV·h-1水洗1 h,再用75%乙醇4 BV,以2 BV·h-1流速进行洗脱,经大孔吸附树脂纯化后的裸花紫珠总黄酮的平均纯度可达47.4%。结论 HP-20型大孔吸附树脂适用于裸花紫珠总黄酮的初步纯化。
목적：우선라화자주총황동적대공흡부수지순화공예。방법이총황동적흡부솔화세탈솔위지표,채용정태흡부우선AB-8、D-101、HP-20、HP2MG등대공흡부수지적형호；통과단인소실험우선라화자주적동태흡부분리삼수。결과 HP-20형수지적흡부효솔교고,기최가적순화공예위 pH 3.0적4.46 mg · mL-1라화자주제취액,이3 BV·h-1류속상양,3 BV·h-1수세1 h,재용75%을순4 BV,이2 BV·h-1류속진행세탈,경대공흡부수지순화후적라화자주총황동적평균순도가체47.4%。결론 HP-20형대공흡부수지괄용우라화자주총황동적초보순화。
Objective To optimize purification technology of total flavonoids in Callicarpa nudiflora Hook. et Arm. by macroporous adsorption resin. Methods Macroporous resin models including AB-8, D-101, HP-20, HP2MG, were optimized by static adsorption and desorption experiments regarding to adsorption rate and desorption rate of total flavonoids. Purification technology parameters of total flavonoids were optimized by single factor test. Results HP-20 macroporous resin presented the best purification efficiency,the optimum purification conditions were that taking 4. 46 mg·mL-1 of total flavonoidsat pH 3. 0, loading at 3 BV·h-1, washed with 3BV of water at 3 BV·h-1,then eluted with 4 BV 75% ethanol at 2 BV·h-1, finally obtaining the total flavonoids from the dry extract of Callicarpa nudiflora Hook. et Arn. with the purity of 47. 4%. Conclusion HP-20 macroporous resin is suitable for preliminary purification of total flavonoids in Callicarpa nudiflora Hook. et Arn.