CAJ | 학술논문

目的：探讨白芍总苷(TGP)对系统性红斑狼疮(SLE)患者外周血B细胞内Toll样受体9(TLR9)表达的影响。方法选取SLE患者60例和正常健康人30例,分离外周血单个核细胞( PBMC),每份标本均分为4组体外培养,即空白对照组、胞嘧啶-磷酸-鸟嘌呤-寡聚脱氧核苷酸( CpG-ODN)组、CpG-ODN+TGP 组和 TGP 组。空白对照组仅加入RPMI-1640完全培养液,其他3组分别加入CpG-ODN( ODN终浓度1μmol·L-1)、CpG-ODN+TPG( TPG最适终浓度为1×10-4 mol·L-1)、TPG。各组均培养48 h后提取细胞,应用流式细胞仪检测各组B细胞内TLR9表达。结果①正常健康人：CpG-ODN组和空白对照组TLR9的表达率分别为(9.10±2.12)%,(4.96±2.11)%(P<0.01)；②SLE患者：CpG-ODN组的TLR9表达率(14.86±3.42)%,显著高于空白对照组的(9.20±3.43)%(P<0.01),CpG-ODN+TGP组的TLR9表达率(11.95±3.63)%,低于CpG-ODN组(P<0.05)；在SLE轻度活动患者中,CpG-ODN组和空白对照组TLR9的表达率分别为(10.74±3.17)%,(5.19±2.05)%(P<0.05)；在SLE中重度活动患者中,CpG-ODN组和空白对照组TLR9的表达率分别为(16.51±1.72)%,(10.80±2.37)%(P<0.01),CpG-ODN+TGP组TLR9的表达率(13.59±2.58)%,低于CpG-ODN组(P<0.01)。结论 TGP可以拮抗CpG-ODN对B细胞内TLR9表达的上调作用。
목적：탐토백작총감(TGP)대계통성홍반랑창(SLE)환자외주혈B세포내Toll양수체9(TLR9)표체적영향。방법선취SLE환자60례화정상건강인30례,분리외주혈단개핵세포( PBMC),매빈표본균분위4조체외배양,즉공백대조조、포밀정-린산-조표령-과취탈양핵감산( CpG-ODN)조、CpG-ODN+TGP 조화 TGP 조。공백대조조부가입RPMI-1640완전배양액,기타3조분별가입CpG-ODN( ODN종농도1μmol·L-1)、CpG-ODN+TPG( TPG최괄종농도위1×10-4 mol·L-1)、TPG。각조균배양48 h후제취세포,응용류식세포의검측각조B세포내TLR9표체。결과①정상건강인：CpG-ODN조화공백대조조TLR9적표체솔분별위(9.10±2.12)%,(4.96±2.11)%(P<0.01)；②SLE환자：CpG-ODN조적TLR9표체솔(14.86±3.42)%,현저고우공백대조조적(9.20±3.43)%(P<0.01),CpG-ODN+TGP조적TLR9표체솔(11.95±3.63)%,저우CpG-ODN조(P<0.05)；재SLE경도활동환자중,CpG-ODN조화공백대조조TLR9적표체솔분별위(10.74±3.17)%,(5.19±2.05)%(P<0.05)；재SLE중중도활동환자중,CpG-ODN조화공백대조조TLR9적표체솔분별위(16.51±1.72)%,(10.80±2.37)%(P<0.01),CpG-ODN+TGP조TLR9적표체솔(13.59±2.58)%,저우CpG-ODN조(P<0.01)。결론 TGP가이길항CpG-ODN대B세포내TLR9표체적상조작용。
Objective To investigate the effect of total glucosides of paeony ( TGP ) on the expression of Toll-like receptor 9 (TLR9) in peripheral blood B lymphocytes of the patients with systemic lupus erythematosus (SLE). Methods Sixty SLE patients and thirty healthy volunteers were enrolled, peripheral blood mononuclear cells ( PBMC) were isolated from blood samples and divided into 4 groups, which were incubated with CpG-ODN(final concentration was 1 μmol·L-1), CpG-ODN+TGP ( TPG final concentration was 1í10-4 mol·L-1 ) ,TGP and RPMI medium ( as the blank control group) for 48 hours, respectively. FLA ( Flow cytometry analysis) was used to detect the expression of TLR9 on peripheral blood B lymphocytes after incubated. Results ①In the health people, TLR9 expression in the group of CpG-ODN was(9. 10±2. 12) %, which was higher than the blank control group(4. 96±2. 11) % (P<0. 01).②In the SLE patients, the TLR9 expression in the group of CpG-ODN was(14. 86±3. 42)% , which was significantly higher than the blank control group(9. 20±3. 43) %(P<0. 01). The TLR9 expression in the group of CpG-ODN+TGP was (11. 95±3. 63)%, which was lower than that in the group of CpG-ODN (P<0. 05). The TLR9 expression in the group of CpG-ODN with lightly active degree SLE patients was (10. 74±3. 17)%, which was higher than the blank control group(5. 19±2. 05) % (P<0. 01). For SLE from the moderately to vigorously active degree, the expression of TLR9 in the group of CpG-ODN(16. 51±1. 72) % was higher than the blank control group(10. 80±2. 37) %(P<0. 01), but that in the group of CpG-ODN+TGP (13. 59±2. 58) % was lower than the group of CpG-ODN (P<0. 01). Conclusion Our data indicate that TGP antagonize upregulation effect of CpG-ODN on the expression of TLR9 in peripheral blood B lymphocytes.