CAJ | 학술논문

背景：骨髓间充质干细胞在癌性微环境中的遗传稳定性问题如何进一步明确并加以有效预防，日益成为推广其临床应用的关键问题。非可控性炎症和肿瘤之间存在互为因果现象，越来越受到科学界关注。目的：分析骨髓间质干细胞在肿瘤微环境中的炎性信号通路及遗传稳定性的变化，并基于中医学温阳固肾、益气升阳、扶正祛邪理论为指导，探讨温阳益气法提高骨髓间质干细胞在肿瘤相关炎性微环境中的效应及其作用机制。方法：以“骨髓间充质干细胞，炎性平衡，补肾，温阳，益气，升阳，肿瘤，癌，阳化气，阴成形，遗传稳定性”为中文检索词，以“bone marrow mesenchymal stem cel s, inflammatory balance, replenishing qi, warming yang, strengthening shen, elevate yang, tumor, cancer, yang can transform qi, yin shaping up body, genetic stability”为英文检索词，应用计算机检索中国知网CNKI数据库、维普和PubMed数据库，严格按纳入标准、排除标准进行文献质量评估和筛选，最终选择42篇文献进行综合分析。结果与结论：在理化因素诱导或肿瘤微环境中，骨髓间充质干细胞可促进肿瘤增殖、侵袭转移，另外骨髓间充质干细胞自身也可被诱发转化为肿瘤相关纤维母细胞，发生遗传稳定性改变。炎性微环境及其骨髓间充质干细胞炎性信号通路异常在骨髓间充质干细胞的遗传稳定性改变中发挥重要作用，基于中医学温阳固肾、益气升阳、扶正祛邪理论，发现温阳益气类中药可明显提高肿瘤微环境及化学癌环境中骨髓间充质干细胞的遗传稳定性。
배경：골수간충질간세포재암성미배경중적유전은정성문제여하진일보명학병가이유효예방，일익성위추엄기림상응용적관건문제。비가공성염증화종류지간존재호위인과현상，월래월수도과학계관주。목적：분석골수간질간세포재종류미배경중적염성신호통로급유전은정성적변화，병기우중의학온양고신、익기승양、부정거사이론위지도，탐토온양익기법제고골수간질간세포재종류상관염성미배경중적효응급기작용궤제。방법：이“골수간충질간세포，염성평형，보신，온양，익기，승양，종류，암，양화기，음성형，유전은정성”위중문검색사，이“bone marrow mesenchymal stem cel s, inflammatory balance, replenishing qi, warming yang, strengthening shen, elevate yang, tumor, cancer, yang can transform qi, yin shaping up body, genetic stability”위영문검색사，응용계산궤검색중국지망CNKI수거고、유보화PubMed수거고，엄격안납입표준、배제표준진행문헌질량평고화사선，최종선택42편문헌진행종합분석。결과여결론：재이화인소유도혹종류미배경중，골수간충질간세포가촉진종류증식、침습전이，령외골수간충질간세포자신야가피유발전화위종류상관섬유모세포，발생유전은정성개변。염성미배경급기골수간충질간세포염성신호통로이상재골수간충질간세포적유전은정성개변중발휘중요작용，기우중의학온양고신、익기승양、부정거사이론，발현온양익기류중약가명현제고종류미배경급화학암배경중골수간충질간세포적유전은정성。
BACKGROUND:How to further definite and prevent the genetic instability of bone marrow mesenchymal stem cel s in tumor microenvironment has increasingly become a key problem for its clinic application. Meanwhile, increasing studies focus on the reciprocal causation relationship between uncontrol ed inflammation and tumors. OBJECTIVE:To analyze the changes of inflammation-related signaling pathways and genetic stability in bone marrow mesenchymal stem cel s under the tumor microenvironment, and meanwhile, to investigate the influence and mechanism of“warming yang and benefiting qi”method on inflammatory signaling pathways and the genetic stability of bone marrow mesenchymal stem cel s in tumor microenvironment, based on the guidance of the traditional Chinese Medicine theories“warming yang and tonifying kidney, benefiting qi and raising yang, strengthening healthy and discarding evil to eliminate pathogens”. METHODS:A computer-based online search of PubMed, CNKI and VIP was performed for relevant articles with the keywords of“bone marrow mesenchymal stem cel s, inflammatory balance, replenishing qi, warming yang, strengthening shen, elevate yang, tumor, cancer, yang can transform qi, yin shaping up body, genetic stability”in English and Chinese, respectively. Screened literatures were evaluated strictly according to the inclusion and exclusion criteria, and final y 42 articles were included in result analysis. RESULTS AND CONCLUSION:Under physical and chemical induction or tumor microenvironment, bone marrow mesenchymal stem cel s can promote tumor proliferation, invasion and metastasis. In addition, bone marrow mesenchymal stem cel s can be induced to differentiate into malignant cel s or tumor associating fibroblasts, fol owed by genetic instability, in tumor microenvironment. Inflammatory microenvironment plays an important role in regulating inflammation-related signals and genetic stability of bone marrow mesenchymal stem cel s. Chinese traditional medicines functioning as“warming yang and replenishing qi”can restore the genetic stability of bone marrow mesenchymal stem cel s in tumor or chemical microenvironment, based on the guidance of the traditional Chinese Medicine theories of“warming yang and tonifying kidney, benefiting qi and raising yang, strengthening healthy and discarding evil to eliminate pathogens”.