酿酒科技
釀酒科技
양주과기
LIQUOR-MAKING SCIENCE & TECHNOLOGY
2015年
5期
56-59
,共4页
白酒%血糖%胰岛素%免疫组织化学%放射免疫法%大鼠
白酒%血糖%胰島素%免疫組織化學%放射免疫法%大鼠
백주%혈당%이도소%면역조직화학%방사면역법%대서
Baijiu(liquor)%blood glucose%insulin%immunohistochemistry%radioimmunoassay%rat
模拟正常人饮酒习惯，探讨摄入某品牌白酒后，不同时长及不同剂量对大鼠血糖、胰岛B细胞表达胰岛素（Ins）及血清Ins的影响。采用正常成年雄性SD大鼠，随机分为正常对照组及实验组。其中实验组分为4周组（4周）、8周组(8周)、12周组（12周）3个时间点；每个时间点又分为低剂量组（L组，0.8 mL/kg·d）、中剂量组（M组，1.6 mL/kg·d）、高剂量组（H组，2.4 mL/kg·d）。实验组大鼠每日11AM及5PM各灌胃1次，正常对照组不予处理。分别于第4周末、第8周末、第12周末测空腹血糖，采集血清，取胰尾组织。采用放射免疫测定法、免疫组化SABC法、图像分析及形态计量法进行研究。结果表明，各实验组大鼠空腹血糖水平及大鼠胰岛面数密度与正常对照组比较，差异无统计学意义（P＞0.05）；与正常对照组比较，除8周（H）组和12周（L）组稍有升高外，其余实验各组血清Ins、胰岛B细胞的免疫染色强度及平均光密度的差异无统计学意义（P＞0.05）。在本实验设定的剂量和时程内用该白酒灌胃，大鼠血糖未见明显异常；对胰岛B细胞表达和面数密度、大鼠血清Ins水平无明显影响。
模擬正常人飲酒習慣，探討攝入某品牌白酒後，不同時長及不同劑量對大鼠血糖、胰島B細胞錶達胰島素（Ins）及血清Ins的影響。採用正常成年雄性SD大鼠，隨機分為正常對照組及實驗組。其中實驗組分為4週組（4週）、8週組(8週)、12週組（12週）3箇時間點；每箇時間點又分為低劑量組（L組，0.8 mL/kg·d）、中劑量組（M組，1.6 mL/kg·d）、高劑量組（H組，2.4 mL/kg·d）。實驗組大鼠每日11AM及5PM各灌胃1次，正常對照組不予處理。分彆于第4週末、第8週末、第12週末測空腹血糖，採集血清，取胰尾組織。採用放射免疫測定法、免疫組化SABC法、圖像分析及形態計量法進行研究。結果錶明，各實驗組大鼠空腹血糖水平及大鼠胰島麵數密度與正常對照組比較，差異無統計學意義（P＞0.05）；與正常對照組比較，除8週（H）組和12週（L）組稍有升高外，其餘實驗各組血清Ins、胰島B細胞的免疫染色彊度及平均光密度的差異無統計學意義（P＞0.05）。在本實驗設定的劑量和時程內用該白酒灌胃，大鼠血糖未見明顯異常；對胰島B細胞錶達和麵數密度、大鼠血清Ins水平無明顯影響。
모의정상인음주습관，탐토섭입모품패백주후，불동시장급불동제량대대서혈당、이도B세포표체이도소（Ins）급혈청Ins적영향。채용정상성년웅성SD대서，수궤분위정상대조조급실험조。기중실험조분위4주조（4주）、8주조(8주)、12주조（12주）3개시간점；매개시간점우분위저제량조（L조，0.8 mL/kg·d）、중제량조（M조，1.6 mL/kg·d）、고제량조（H조，2.4 mL/kg·d）。실험조대서매일11AM급5PM각관위1차，정상대조조불여처리。분별우제4주말、제8주말、제12주말측공복혈당，채집혈청，취이미조직。채용방사면역측정법、면역조화SABC법、도상분석급형태계량법진행연구。결과표명，각실험조대서공복혈당수평급대서이도면수밀도여정상대조조비교，차이무통계학의의（P＞0.05）；여정상대조조비교，제8주（H）조화12주（L）조초유승고외，기여실험각조혈청Ins、이도B세포적면역염색강도급평균광밀도적차이무통계학의의（P＞0.05）。재본실험설정적제량화시정내용해백주관위，대서혈당미견명현이상；대이도B세포표체화면수밀도、대서혈청Ins수평무명현영향。
In this study, simulating liquor drinking habits of normal people, we explored the expression changes of insulin in pancreatic islet B cells and serum insulin levels in rats after consumption Baijiu(liquor) of different time period and different dose. Normal male adult SD rats were randomly divided into control group and experiment groups. Experiment groups included 4 weeks group(4w), 8 weeks group(8w) and 12 weeks group(12w);each group were further divided into low dose group (L, 0.8 mL/kg·d), middle dose group (M, 1.6 mL/kg·d) and high dose group (H, 2.4 mL/kg·d). Rats in experiment groups were given by gavage twice a day(11 AM and 5 PM) with Baijiu(liquor), while rats in con-trol group received no special treatment. At the end of the 4th, 8th and 12th week, the rats’fasting blood glucose was measured, serum samples and pancreatic tail tissue were collected. Samples were analyzed by radioimmunoassay, immunohistochemical SABC, image analysis and mor-phometry methods. The results showed that, there was no statistically significant difference in fasting blood glucose level and pancreatic islet numerical density on area among experiment groups and control group (P>0.05). Compared with control group, the immune staining intensity of pancreatic islet B cells and serum insulin levels of 8w(H) group and 12w (L) group increased slightly(P<0.05). The treatment of gavage with 54%vol Baijiu(liquor) at the dosage and time period in this experiment had no significant effect on fasting blood glucose, immune staining in-tensity and numerical density on area of pancreatic islet B cells, and serum insulin levels of rats. (Trans. by HUANG Xiaoli)
