CAJ | 학술논문

对从浓香型大曲中提取酯化酶的工艺条件进行了研究。研究了几种不同介质对酯化酶提取酶酶活的影响，确定了采用NaCl溶液进行提取时，酶活最高；通过单因素法分析了提取时间、提取液浓度、提取温度、固液比及pH值5个因素对提取酶活的影响，再在单因素试验结果的基础上，以比酶活力为指标进行L9(34)正交试验，最后得到的最佳工艺条件为：提取温度4℃，提取固液比1∶5，提取时间1 h，NaCl浓度0.3 mol/L，NaCl溶液pH6。在此条件下酶液的平均比酶活力为28.74 U/mg。
대종농향형대곡중제취지화매적공예조건진행료연구。연구료궤충불동개질대지화매제취매매활적영향，학정료채용NaCl용액진행제취시，매활최고；통과단인소법분석료제취시간、제취액농도、제취온도、고액비급pH치5개인소대제취매활적영향，재재단인소시험결과적기출상，이비매활력위지표진행L9(34)정교시험，최후득도적최가공예조건위：제취온도4℃，제취고액비1∶5，제취시간1 h，NaCl농도0.3 mol/L，NaCl용액pH6。재차조건하매액적평균비매활력위28.74 U/mg。
The technical conditions of the extraction of esterifying enzyme from Nongxiang Daqu were studied. Firstly, NaCl solution was de-termined as the extraction medium among different extraction mediums because enzyme activity extracted by NaCl solution was the highest. Then the effects of five factors including extracting time, extracting solution concentration, extracting temperature, the ratio of solid to liquid and pH value on the activity of esterifying enzyme were investigated by single factor test. On the basis of single factor experiment, L9(34) or-thogonal test was done with specific activity of esterifying enzyme as the indicator. Finally, the optimum extracting technical conditions were obtained as follows:the extracting temperature was at 4℃, the ratio of solid to liquid was 1∶5, the extracting time was 1 h, the concentration of NaCl solution was 0.3 mol/L, and pH value of NaCl solution was 6. Under the above conditions, the specific activity of enzyme reached an av-erage of 28.74 U/mg.