CAJ | 학술논문

背景：人类胚胎干细胞体外建系成功，对人类胚胎发育机制和发育生物学研究、细胞和组织移植治疗某些疾病等领域都有重大意义。目的：综述近年来关于胚胎体外培养及建立胚胎干细胞系的研究进展，重点探讨胚胎体外培养影响因素、人废弃胚胎培养分离内细胞团建立胚胎干细胞系的方法及建立胚胎干细胞系的条件。方法：以“胚胎(embryo)，胚胎干细胞(embryonic stem cel )，共培养(co culture)，序贯培养(sequential culture)”为检索词，由第一作者检索2000至2014年CNKI数据库和SCI数据库，获取有关胚胎体外培养、移植及胚胎干细胞建系的相关文献，并进行系统评价，最终保留58篇文献进行分析。结果与结论：胚胎体外培养条件是影响胚胎移植结局的重要因素，其中包括培养液的成分和培养体系。在过去的研究过程中，培养液的构成及应用已经发生了很大的变化，培养体系也从单一培养发展到共培养、序贯培养。伦理问题及胚胎来源的限制束缚着人胚胎干细胞系的建立，利用临床废弃的低质量的胚胎可作为建立人胚胎干细胞系的材料来源之一，有效的缓解了建立人胚胎干细胞系过程中胚胎缺乏的问题，并减少其中的伦理学纷争。
배경：인류배태간세포체외건계성공，대인류배태발육궤제화발육생물학연구、세포화조직이식치료모사질병등영역도유중대의의。목적：종술근년래관우배태체외배양급건립배태간세포계적연구진전，중점탐토배태체외배양영향인소、인폐기배태배양분리내세포단건립배태간세포계적방법급건립배태간세포계적조건。방법：이“배태(embryo)，배태간세포(embryonic stem cel )，공배양(co culture)，서관배양(sequential culture)”위검색사，유제일작자검색2000지2014년CNKI수거고화SCI수거고，획취유관배태체외배양、이식급배태간세포건계적상관문헌，병진행계통평개，최종보류58편문헌진행분석。결과여결론：배태체외배양조건시영향배태이식결국적중요인소，기중포괄배양액적성분화배양체계。재과거적연구과정중，배양액적구성급응용이경발생료흔대적변화，배양체계야종단일배양발전도공배양、서관배양。윤리문제급배태래원적한제속박착인배태간세포계적건립，이용림상폐기적저질량적배태가작위건립인배태간세포계적재료래원지일，유효적완해료건립인배태간세포계과정중배태결핍적문제，병감소기중적윤리학분쟁。
BACKGROUND:The successful establishment of human embryonic stem cel lines in vitro is of great significance to human embryonic development mechanism and developmental biology, cel and tissue transplantation in the treatment of certain diseases. OBJECTIVE:To summarize the progress of in vitro culture of embryos and establishment of embryonic stem cel lines, to explore the influential factors for in vitro culture of embryos, and the methods of culturing human discarded embryos, isolating inner cel mass and establishing embryonic stem cel lines, as wel as the establishing conditions for embryonic stem cel lines. METHODS:With the key words of“embryo, embryonic stem cel s, coculture, sequential culture”, the first author searched CNKI and SCI databases for literatures concerning in vitro culture and transplantation of embryos and establishment of embryonic stem cel lines published from 2000 to 2014. Systematic evaluation was conducted. Final y, 58 literatures were retained for result analysis. RESULTS AND CONCLUSION:The culturing condition for embryos in vitro is the key factor affecting embryo transfer outcomes, including culture medium component and culture system. In previous studies, the component and application of culture medium have changed greatly, and the culture system has altered from single culture to coculture and sequential culture. Ethical issues and embryonic origin restrictions restrict the establishment of human embryonic stem cel lines. Clinical y discarded low-quality embryos can be used as one of the material sources to establish human embryonic stem cel lines, which can effectively lessen the problem of embryo shortage during the establishment of human embryonic stem cel lines and reduce ethical disputes.