CAJ | 학술논문

背景：镁黄长石属于硅酸盐生物活性陶瓷，在生物体内具有降解作用，降解溶出的离子产物能够诱导细胞成骨分化，是组织工程骨支架材料的良好选择。目的：通过研究不同浓度的镁黄长石浸提液对诱导性多潜能干细胞增殖和成骨分化的影响，确定镁黄长石浸提液促进诱导性多潜能干细胞成骨分化的最佳浓度。方法：将诱导性多潜能干细胞培养于不同浓度的镁黄长石浸提液中，用MTT法检测细胞的增殖情况；在第7，14，21天时，分别收集培养上清液，检测其中碱性磷酸酶、骨钙素、Ⅰ型胶原蛋白含量。结果与结论：镁黄长石浸提液促进诱导性多潜能干细胞增殖的作用具有时间依赖性，第3天时，诱导性多潜能干细胞增殖明显，第5天与第3天相比差异无显著性意义，至第7天时则明显减弱。同时，1/4浓度浸提液组促进诱导性多潜能干细胞增殖作用最强。作为细胞成骨分化标志的碱性磷酸酶和骨钙素含量随时间增加而增加，1/4浸提液浓度组含量最高。作为细胞成骨分化早中期标志的Ⅰ型胶原蛋白在第7天时各组均未检出，第14，21天时，同样是1/4浓度浸提液组含量最高，这些说明镁黄长石浸提液中的Ca、Mg、Si离子参与了诱导性多潜能干细胞的成骨分化，其中1/4浓度浸提液(Ca离子2.37 mmol/L、Mg离子1.12 mmol/L、Si离子1.05 mmol/L)促进诱导性多潜能干细胞体外成骨分化的效果最好。
배경：미황장석속우규산염생물활성도자，재생물체내구유강해작용，강해용출적리자산물능구유도세포성골분화，시조직공정골지가재료적량호선택。목적：통과연구불동농도적미황장석침제액대유도성다잠능간세포증식화성골분화적영향，학정미황장석침제액촉진유도성다잠능간세포성골분화적최가농도。방법：장유도성다잠능간세포배양우불동농도적미황장석침제액중，용MTT법검측세포적증식정황；재제7，14，21천시，분별수집배양상청액，검측기중감성린산매、골개소、Ⅰ형효원단백함량。결과여결론：미황장석침제액촉진유도성다잠능간세포증식적작용구유시간의뢰성，제3천시，유도성다잠능간세포증식명현，제5천여제3천상비차이무현저성의의，지제7천시칙명현감약。동시，1/4농도침제액조촉진유도성다잠능간세포증식작용최강。작위세포성골분화표지적감성린산매화골개소함량수시간증가이증가，1/4침제액농도조함량최고。작위세포성골분화조중기표지적Ⅰ형효원단백재제7천시각조균미검출，제14，21천시，동양시1/4농도침제액조함량최고，저사설명미황장석침제액중적Ca、Mg、Si리자삼여료유도성다잠능간세포적성골분화，기중1/4농도침제액(Ca리자2.37 mmol/L、Mg리자1.12 mmol/L、Si리자1.05 mmol/L)촉진유도성다잠능간세포체외성골분화적효과최호。
BACKGROUND:Akermanite belongs to silicate bioactive ceramics, with degradation in vivo, and its ionic products can induce osteogenic differentiation, which is a good choice for bone tissue engineering scaffold. OBJECTIVE:By studying the different concentrations of akermanite extracts on the proliferation and osteogenic differentiation of pluripotent stem cel s, to determine the optimum concentration of akermanite extract to inducing the osteogenic differentiation of pluripotent stem cel s. METHODS:MTT assay was used to detect the proliferation of pluripotent stem cel s cultured in different concentrations of akermanite extracts (1/2, 1/4, 1/8, 1/16, 1/32). After cultured with the extracts for 7, 14, 21 days, the culture supernatants were col ected to detect the levels of alkaline phosphatase, osteocalcin, type I col agen. RESULTS AND CONCLUSION:The MTT assay showed that the proliferation of pluripotent stem cel s was increased in a concentration-dependent manner after induction with akermanite extracts. The pluripotent stem cel s proliferated obviously at 3 days after induction, and then weaken at 7 days, but there was no difference at 3 and 5 days after induction. At 7 days after induction, the 1/4 extract had the best effect on promoting osteogenic differentiation of pluripotent stem cel s;the levels of alkaline phosphatase and osteocalcin were increased with time, especial y after induction with the 1/4 extract;but there was no expression of type I col agen. At 14 and 21 days after induction, the levels of alkaline phosphatase, osteocalcin, type I col agen were highest in the 1/4 extract group. These findings indicate that the 1/4 akermanite extract (Ca 2.37 mmol/L, Mg 1.12 mmol/L, Si 1.05 mmol/L) is the optimum to promote the osteogenic differentiation of pluripotent stem cel s.