CAJ | 학술논문

背景：近年来研究资料显示，糖尿病并发症的发生、发展与机体内氧化应激的平衡破坏程度有着密切的相关性。目的：探讨胚胎干细胞对糖尿病肾病大鼠氧化应激反应的影响。方法：原代培养小鼠胚胎干细胞，观察细胞形态，免疫组化检测表面抗原标志表达。腹腔注射柠檬酸钠-柠檬酸缓冲液稀释的链脲佐菌素建立糖尿病肾病SD大鼠模型，细胞实验组经尾静脉注射胚胎干细胞，模型对照组注射等量PBS，正常对照组不造模，一次性腹腔注射柠檬酸钠-柠檬酸缓冲液，共注射2次，末次注射细胞后5周，检测各组大鼠血糖、肾功能指标(尿蛋白/尿肌酐、血尿素氮和血肌酐)，测定肾组织氧化应激产物丙二醛及蛋白羰基含量，免疫印迹检测超氧化物歧化酶的表达水平。结果与结论：胚胎干细胞为椭圆形或圆形，边界清晰，折光性好，高表达Oct-4、SSEA-1；与正常对照组比较，模型对照组和细胞实验组肾功生化指标、丙二醛、蛋白羰基含量均明显升高，超氧化物歧化酶表达量显著下降(P<0.05)；与模型对照组比较，细胞实验组肾功生化指标、丙二醛、蛋白羰基含量明显下调，超氧化物歧化酶表达量明显回升(P<0.05)。结果表明胚胎干细胞可以通过抑制氧化应激反应的进程从而逆转糖尿病肾病的发生发展。
배경：근년래연구자료현시，당뇨병병발증적발생、발전여궤체내양화응격적평형파배정도유착밀절적상관성。목적：탐토배태간세포대당뇨병신병대서양화응격반응적영향。방법：원대배양소서배태간세포，관찰세포형태，면역조화검측표면항원표지표체。복강주사저몽산납-저몽산완충액희석적련뇨좌균소건립당뇨병신병SD대서모형，세포실험조경미정맥주사배태간세포，모형대조조주사등량PBS，정상대조조불조모，일차성복강주사저몽산납-저몽산완충액，공주사2차，말차주사세포후5주，검측각조대서혈당、신공능지표(뇨단백/뇨기항、혈뇨소담화혈기항)，측정신조직양화응격산물병이철급단백탄기함량，면역인적검측초양화물기화매적표체수평。결과여결론：배태간세포위타원형혹원형，변계청석，절광성호，고표체Oct-4、SSEA-1；여정상대조조비교，모형대조조화세포실험조신공생화지표、병이철、단백탄기함량균명현승고，초양화물기화매표체량현저하강(P<0.05)；여모형대조조비교，세포실험조신공생화지표、병이철、단백탄기함량명현하조，초양화물기화매표체량명현회승(P<0.05)。결과표명배태간세포가이통과억제양화응격반응적진정종이역전당뇨병신병적발생발전。
BACKGROUND:Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body. OBJECTIVE:To investigate the effect of embryonic stem cel s on oxidative stress response of rats with diabetic nephropathy. METHODS:Primarily cultured rat embryonic stem cel s were observed for cel morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cel s via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneal y injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group;contents of malondialdehyde and protein carbonyl were detected in the kidney;the expression level of superoxide dismutase was detected by western blot assay. RESULTS AND CONCLUSION:The embryonic stem cel s were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatical y in the model group and experimental group (P<0.05);compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P<0.05). Taken together, embryonic stem cel s can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.