CAJ | 학술논문

背景：目前，来源于人脐带的间充质干细胞移植途径主要为局部移植，存在创伤大、出血多、并发症多等缺点，限制了其在临床上的广泛应用。目的：探讨静脉移植人脐带间充质干细胞治疗脊髓损伤的可行性。方法：80只Wistar大鼠成功制备脊髓打击模型，随机分为5组：空白对照组10只大鼠不进行任何移植；DMEM局部移植组15只、DMEM静脉移植组15只大鼠分别通过局部、静脉途径移植DMEM；细胞局部移植组20只、细胞静脉移植组20只大鼠分别通过局部、静脉途径移植人脐带间充质干细胞悬液。术后定期行BBB评分、组织学观察和免疫组化检测来评价脊髓修复情况。结果与结论：移植后1 d-2周各组间BBB评分差距小，差异无显著性意义；移植后4-12周，细胞局部移植组、细胞静脉移植组BBB评分明显高于其他3组，差异有显著性意义；细胞局部移植组、细胞静脉移植组间差异无显著性意义(P>0.05)。组织学观察显示，细胞局部移植组、细胞静脉移植组损伤局部形成的空洞及胶质瘢痕较其他3组都相对减少，而细胞静脉移植组和细胞局部移植组相近。以上结果表明静脉移植的人脐带间充质干细胞能迁移到脊髓损伤部位，对急性脊髓损伤产生修复作用，且效果与局部移植相似。该方式简单易行，避免二次损伤、感染等，为治疗脊髓损伤提供了一种新的细胞移植途径。
배경：목전，래원우인제대적간충질간세포이식도경주요위국부이식，존재창상대、출혈다、병발증다등결점，한제료기재림상상적엄범응용。목적：탐토정맥이식인제대간충질간세포치료척수손상적가행성。방법：80지Wistar대서성공제비척수타격모형，수궤분위5조：공백대조조10지대서불진행임하이식；DMEM국부이식조15지、DMEM정맥이식조15지대서분별통과국부、정맥도경이식DMEM；세포국부이식조20지、세포정맥이식조20지대서분별통과국부、정맥도경이식인제대간충질간세포현액。술후정기행BBB평분、조직학관찰화면역조화검측래평개척수수복정황。결과여결론：이식후1 d-2주각조간BBB평분차거소，차이무현저성의의；이식후4-12주，세포국부이식조、세포정맥이식조BBB평분명현고우기타3조，차이유현저성의의；세포국부이식조、세포정맥이식조간차이무현저성의의(P>0.05)。조직학관찰현시，세포국부이식조、세포정맥이식조손상국부형성적공동급효질반흔교기타3조도상대감소，이세포정맥이식조화세포국부이식조상근。이상결과표명정맥이식적인제대간충질간세포능천이도척수손상부위，대급성척수손상산생수복작용，차효과여국부이식상사。해방식간단역행，피면이차손상、감염등，위치료척수손상제공료일충신적세포이식도경。
BACKGROUND:Currently, human umbilical cord derived-mesenchymal stem cel s are mainly for local transplantation, which has some shortcomings, such as large trauma, bleeding, complications, that limit its widespread application in clinical practice. OBJECTIVE:To investigate the feasibility of intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s for repair of spinal cord injury. METHODS:Eighty Wistar rats with spinal cord hitting were divided into five groups:blank control group with no transplantation (n=10), DMEM local transplantation group (n=15), DMEM intravenous transplantation group (n=15), cel local transplantation group (n=20), cel intravenous transplantation group (n=20). The functional recovery of spinal cord injury was observed with Basso, Beattie and Bresnahan scores at regular time as wel as hematoxylin-eosin staining and immunohistochemistry staining. RESULTS AND CONCLUSION:During 1 day to 2 weeks after transplantation, there was no significant difference in the Basso, Beattie and Bresnahan scores between the five groups;within 4-12 weeks after transplantation, the Basso, Beattie and Bresnahan scores were significantly higher in the two cel transplantation groups than the other three groups, but there was no difference between these two cel transplantation groups (P>0.05). Histological observation showed that the number of voids and glial scars was less in the cel local transplantation group and cel intravenous transplantation group compared with the other three groups, and there was also no difference between the two cel transplantation groups. These results indicate that the intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s is similar to the local transplantation in the repair of acute spinal cord injury, which is simple and avoids secondary injuries and various complications. It is recommended that this method provide a new approach for cel transplantation.