中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
14期
2179-2185
,共7页
姜艳杰%毛成刚%宁险峰%李荣%李自普
薑豔傑%毛成剛%寧險峰%李榮%李自普
강염걸%모성강%저험봉%리영%리자보
干细胞%移植%扩张型心肌病%人脐带间充质干细胞%肌肉注射%血管内皮生长因子%肝细胞生长因子%胰岛素样生长因子1%粒细胞集落刺激生物因子%白血病抑制因子
榦細胞%移植%擴張型心肌病%人臍帶間充質榦細胞%肌肉註射%血管內皮生長因子%肝細胞生長因子%胰島素樣生長因子1%粒細胞集落刺激生物因子%白血病抑製因子
간세포%이식%확장형심기병%인제대간충질간세포%기육주사%혈관내피생장인자%간세포생장인자%이도소양생장인자1%립세포집락자격생물인자%백혈병억제인자
Umbilical Cord%Mesenchymal Stem Cell Transplantation%Injections,Intramuscular%Cardiomyopathy,Dilated
背景：已有研究表明，在一定的剂量范围内，健康大鼠肌肉注射异种脐带间充质干细胞安全可靠，也证实了这一途径对扩张型心肌病大鼠心力衰竭同样安全有效。目的：探讨肌肉注射脐带间充质干细胞对扩张型心肌病大鼠相关细胞因子表达的影响。方法：雄性SD大鼠160只，随机抽取20只作为正常组，余大鼠通过阿霉素腹腔注射方式建立扩张型心肌病模型。造模成功大鼠随机分为模型组、上清液祖、低剂量组、中剂量组、高剂量组，分别经四肢肌肉注射人脐带间充质干细胞培养液、上清液及不同剂量的人脐带间充质干细胞，注射后观察大鼠一般情况，并于首次注射后4周再次给予相同剂量的培养液、上清液或脐带间充质干细胞。结果与结论：ELISA 检测结果显示，肌注前后模型组大鼠血清肝细胞生长因子、白血病抑制因子、粒细胞集落刺激生物因子及血管内皮生长因子水平均较正常组增高(P<0.05)，肌注后低剂量组肝细胞生长因子、白血病抑制因子、血管内皮生长因子和粒细胞集落刺激生物因子较肌注前显著升高(P<0.05)，且较模型组明显升高(P <0.05)，中剂量组白血病抑制因子较肌注前明显升高(P <0.05)，高剂量组肝细胞生长因子、血管内皮生长因子和粒细胞集落刺激生物因子较肌注前无明显差异(P>0.05)。免疫组化及RT-PCR结果均显示，各肌肉注射组大鼠心肌细胞胰岛素样生长因子1、血管内皮生长因子、肝细胞生长因子均较正常组表达增强，中剂量组呈强阳性表达，较其他各组增强明显。以上结果表明中、低剂量人脐带间充质干细胞肌肉注射可使扩张型心肌病大鼠血清肝细胞生长因子、白血病抑制因子、粒细胞集落刺激生物因子及血管内皮生长因子水平增高，并增加心肌组织胰岛素样生长因子1、肝细胞生长因子、血管内皮生长因子表达。
揹景：已有研究錶明，在一定的劑量範圍內，健康大鼠肌肉註射異種臍帶間充質榦細胞安全可靠，也證實瞭這一途徑對擴張型心肌病大鼠心力衰竭同樣安全有效。目的：探討肌肉註射臍帶間充質榦細胞對擴張型心肌病大鼠相關細胞因子錶達的影響。方法：雄性SD大鼠160隻，隨機抽取20隻作為正常組，餘大鼠通過阿黴素腹腔註射方式建立擴張型心肌病模型。造模成功大鼠隨機分為模型組、上清液祖、低劑量組、中劑量組、高劑量組，分彆經四肢肌肉註射人臍帶間充質榦細胞培養液、上清液及不同劑量的人臍帶間充質榦細胞，註射後觀察大鼠一般情況，併于首次註射後4週再次給予相同劑量的培養液、上清液或臍帶間充質榦細胞。結果與結論：ELISA 檢測結果顯示，肌註前後模型組大鼠血清肝細胞生長因子、白血病抑製因子、粒細胞集落刺激生物因子及血管內皮生長因子水平均較正常組增高(P<0.05)，肌註後低劑量組肝細胞生長因子、白血病抑製因子、血管內皮生長因子和粒細胞集落刺激生物因子較肌註前顯著升高(P<0.05)，且較模型組明顯升高(P <0.05)，中劑量組白血病抑製因子較肌註前明顯升高(P <0.05)，高劑量組肝細胞生長因子、血管內皮生長因子和粒細胞集落刺激生物因子較肌註前無明顯差異(P>0.05)。免疫組化及RT-PCR結果均顯示，各肌肉註射組大鼠心肌細胞胰島素樣生長因子1、血管內皮生長因子、肝細胞生長因子均較正常組錶達增彊，中劑量組呈彊暘性錶達，較其他各組增彊明顯。以上結果錶明中、低劑量人臍帶間充質榦細胞肌肉註射可使擴張型心肌病大鼠血清肝細胞生長因子、白血病抑製因子、粒細胞集落刺激生物因子及血管內皮生長因子水平增高，併增加心肌組織胰島素樣生長因子1、肝細胞生長因子、血管內皮生長因子錶達。
배경：이유연구표명，재일정적제량범위내，건강대서기육주사이충제대간충질간세포안전가고，야증실료저일도경대확장형심기병대서심력쇠갈동양안전유효。목적：탐토기육주사제대간충질간세포대확장형심기병대서상관세포인자표체적영향。방법：웅성SD대서160지，수궤추취20지작위정상조，여대서통과아매소복강주사방식건립확장형심기병모형。조모성공대서수궤분위모형조、상청액조、저제량조、중제량조、고제량조，분별경사지기육주사인제대간충질간세포배양액、상청액급불동제량적인제대간충질간세포，주사후관찰대서일반정황，병우수차주사후4주재차급여상동제량적배양액、상청액혹제대간충질간세포。결과여결론：ELISA 검측결과현시，기주전후모형조대서혈청간세포생장인자、백혈병억제인자、립세포집락자격생물인자급혈관내피생장인자수평균교정상조증고(P<0.05)，기주후저제량조간세포생장인자、백혈병억제인자、혈관내피생장인자화립세포집락자격생물인자교기주전현저승고(P<0.05)，차교모형조명현승고(P <0.05)，중제량조백혈병억제인자교기주전명현승고(P <0.05)，고제량조간세포생장인자、혈관내피생장인자화립세포집락자격생물인자교기주전무명현차이(P>0.05)。면역조화급RT-PCR결과균현시，각기육주사조대서심기세포이도소양생장인자1、혈관내피생장인자、간세포생장인자균교정상조표체증강，중제량조정강양성표체，교기타각조증강명현。이상결과표명중、저제량인제대간충질간세포기육주사가사확장형심기병대서혈청간세포생장인자、백혈병억제인자、립세포집락자격생물인자급혈관내피생장인자수평증고，병증가심기조직이도소양생장인자1、간세포생장인자、혈관내피생장인자표체。
BACKGROUND:Studies have shown that intramuscular transplantation of xenogeneic umbilical cord mesenchymal stem cel s in a certain dose range is safe and reliable, and it also confirm that this approach is equal y safe and effective for heart failure in rats with dilated cardiomyopathy. OBJECTIVE:To explore the effect of human umbilical cord mesenchymal stem cel s through intramuscular injection on the cytokine expression in adriamycin-induced dilated cardiomyopathy (DCM) rats. METHODS:Total y 160 rats were randomly divided into control group (n=20) and DCM group (n=140). Rats in the DCM group were administered adriamycin intraperitoneal y to establish DCM model. The DCM rats were randomly subdivided into model control group (served as model group), cel supernatant group, the low-dose mesenchymal stem cel group (served as low-dose group), the middle-dose mesenchymal stem cel group (served as middle-dose group), and the high-dose mesenchymal stem cel s group (served as high-dose group). Secondary injection was performed at 4 weeks after first injection. RESULTS AND CONCLUSION:The ELISA test showed that the serum levels of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), leukemia inhibitor factor (LIF) and granulocyte macrophage colony stimulating factor (GM-CSF) were higher in the model group than the control group before and after intramuscular injection (P<0.05). After intramuscular injection, the levels of HGF, LIF, GM-CSF and VEGF in the low-dose group were increased significantly (P<0.05), which were significantly higher than those in the model group (P<0.05). The level of LIF in the middle-dose group was significantly elevated after injection (P<0.05), while there were no significant differences in HGF, VEGF and GM-CSF levels in the high-dose group before and after intramuscular injection (P>0.05). Both the immunohistochemical and RT-PCR results showed that the expressions of insulin-like growth factor-1, VEGF and HGF were increased in al the DCM rats as compared with the control group, which were increased most in the middle-dose group. These findings indicate that low-dose and middle-dose human umbilical cord mesenchymal stem cel s intramuscular injection can increase the serum levels of HGF, LIF, GM-CSF, VEGF and the expressions of IGF-1, HGF and VEGF in the myocardium of DCM rats.
