CAJ | 학술논문

背景：肿瘤复发的原因是具有自我更新特性的肿瘤干细胞未能彻底清除，于是如何标记并消灭肿瘤干细胞成为治疗肿瘤的关键所在。目的：观察结直肠癌组织中CD44+/C-myc+方法：选取150例结直肠腺癌患者的病理组织制成组织芯片，采用免疫组化双重染色观察并计数CD44细胞的形态分布及数量，并探讨其表达与患者肿瘤复发转移的关系。+/C-myc+细胞，并与正常组织、腺瘤组织比较。使用手机、书信等方式对患者进行随访，统计患者的复发转移与CD44+/C-myc+结果与结论：CD44细胞表达的关系。+/C-myc+细胞在正常组织中无分布，在腺瘤组织中极少散在分布，在腺癌组织中少量单个点状或灶性分布，双阳细胞数量与腺癌分化程度、浸润深度、淋巴结转移有关(P<0.05)。单因素分析患者腺癌的总生存率、无进展生存率均与双阳性细胞数量、淋巴结有无转移、Ducks分期有关(P<0.05)，多因素分析患者的总生存率与双阳性细胞数有关，无进展生存率与双阳性细胞数及Ducks分期有关。结果表明结直肠组织中CD44+/C-myc+细胞极有可能为腺癌干细胞；Ducks分期、CD44+/C-myc+细胞的数量是影响患者预后的重要因素。
배경：종류복발적원인시구유자아경신특성적종류간세포미능철저청제，우시여하표기병소멸종류간세포성위치료종류적관건소재。목적：관찰결직장암조직중CD44+/C-myc+방법：선취150례결직장선암환자적병리조직제성조직심편，채용면역조화쌍중염색관찰병계수CD44세포적형태분포급수량，병탐토기표체여환자종류복발전이적관계。+/C-myc+세포，병여정상조직、선류조직비교。사용수궤、서신등방식대환자진행수방，통계환자적복발전이여CD44+/C-myc+결과여결론：CD44세포표체적관계。+/C-myc+세포재정상조직중무분포，재선류조직중겁소산재분포，재선암조직중소량단개점상혹조성분포，쌍양세포수량여선암분화정도、침윤심도、림파결전이유관(P<0.05)。단인소분석환자선암적총생존솔、무진전생존솔균여쌍양성세포수량、림파결유무전이、Ducks분기유관(P<0.05)，다인소분석환자적총생존솔여쌍양성세포수유관，무진전생존솔여쌍양성세포수급Ducks분기유관。결과표명결직장조직중CD44+/C-myc+세포겁유가능위선암간세포；Ducks분기、CD44+/C-myc+세포적수량시영향환자예후적중요인소。
BACKGROUND:Tumor recurrence results from the incomplete removal of cancer stem cel s with self-renewal characteristics, and then how to label and eliminate cancer stem cel s becomes the key to cancer treatment. OBJECTIVE:To observe the morphology,distribution and number of CD44+/C-myc+METHODS:Pathological tissues from 150 patients with colorectal cancer were taken to prepare tissue microarray, in order to observe and count CD44 cel s in colorectal cancer, and to explore the relationship between the expression and postoperative metastasis.+/C-myc+cel s by using immunohistochemical double staining. Patients were fol owed up through mobile phones , letters , and so on, and the relationship between the expression of CD44+/C-myc+cel s and postoperative metastasis were statistical y recorded.RESULTS AND CONCLUSION:There was no CD44+/C-myc+cel s in normal tissue and little in adenoma. A smal number of CD44+/C-myc+cel s distributed as dots or focal lesions in adenocarcinoma. The number of CD44+/C-myc+cel s was related to the degree of adenocarcinoma differentiation, depth of invasion, and lymph node metastasis (P<0.05). The univariate analysis showed that the overal survival rate and progression-free survival rate were associated with the number of CD44+/C-myc+cel s, lymph node metastasis and Ducks staging in adenocarcinoma (P<0.05). The multivariate analysis showed that the overal survival rate was related to CD44+/C-myc+cel amount and the progression-free survival rate was related to CD44+/C-myc+cel amount and the Ducks staging. Therefore, CD44+/C-myc+cel s are probably cancer stem cel s, and Ducks staging and CD44+/C-myc+cel amount are important prognostic factors for colorectal cancer.