CAJ | 학술논문

背景：富血小板血浆含有多种刺激因子，且能上调蛋白多糖及胶原合成。目的：观察山羊富血小板血浆对山羊骨髓间充质干细胞增殖的影响。方法：抽取6月龄的内蒙古锡盟山羊颈静脉血，采用二度离心法获取富血小板血浆，运用骨髓穿刺包从山羊髂骨上抽取骨髓，采用密度梯度离心方法分离、纯化获得山羊骨髓间充质干细胞，然后取生长状态较好的原代细胞，加入含体积分数为10%，20%，30%富血小板血浆的 L-DMEM 完全培养基进行培养，对照组只用L-DMEM完全培养基培养。结果与结论：在2-6d期间，富血小板血浆各组明显比对照组细胞增殖迅速，而且体积分数越高细胞生长的速度越快，生长的数目越多，细胞形态越成熟。培养第4天时，体积分数为10%，20%，30%富血小板血浆组细胞倍增时间约为50 h，35 h，25 h。结果表明山羊富血小板血浆能明显促进骨髓间充质干细胞的增殖，而且富血小板血浆体积分数越大促增殖能力越显著。
배경：부혈소판혈장함유다충자격인자，차능상조단백다당급효원합성。목적：관찰산양부혈소판혈장대산양골수간충질간세포증식적영향。방법：추취6월령적내몽고석맹산양경정맥혈，채용이도리심법획취부혈소판혈장，운용골수천자포종산양가골상추취골수，채용밀도제도리심방법분리、순화획득산양골수간충질간세포，연후취생장상태교호적원대세포，가입함체적분수위10%，20%，30%부혈소판혈장적 L-DMEM 완전배양기진행배양，대조조지용L-DMEM완전배양기배양。결과여결론：재2-6d기간，부혈소판혈장각조명현비대조조세포증식신속，이차체적분수월고세포생장적속도월쾌，생장적수목월다，세포형태월성숙。배양제4천시，체적분수위10%，20%，30%부혈소판혈장조세포배증시간약위50 h，35 h，25 h。결과표명산양부혈소판혈장능명현촉진골수간충질간세포적증식，이차부혈소판혈장체적분수월대촉증식능력월현저。
BACKGROUND:Platelet-rich plasma contains a variety of stimulating factors, and can also raise the proteoglycan and col agen synthesis. OBJECTIVE:To observe the effect of platelet-rich plasma on the proliferation of goat bone marrow mesenchymal stem cel s. METHODS:Blood samples were extracted from the jugular vein of Inner Mongolia Ximeng goats to harvest platelet-rich plasma using centrifugation method. Then, bone marrow was extracted from the goat’s ilium by puncture method to isolate and purify goat bone marrow mesechymal stem cel s using density gradient centrifugation method. After that, primary cel s at good state were cultured in L-DMEM complete medium containing 10%, 20%, 30%platelet-rich plasma or in simple L-DMEM complete medium. RESULTS AND CONCLUSION:Within 2-6 days of culture, cel s in the platelet-rich plasma groups proliferated faster than those in the control group, and with the increasing of platelet-rich plasma concentration, the cel s grew faster, with larger number and more mature morphology. At 4 days of culture, the cel doubling time was about 50, 35, 25 hours in the 10%, 20%, and 30%platelet-rich plasma groups, respectively. These findings indicate that goat platelet-rich plasma can dramatical y promote the proliferation of bone marrow mesenchymal stem cel s in a concentration-dependent manner.