中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
14期
2138-2143
,共6页
景彩霞%刘昌奎%谭新颖%罗金超%胡敏
景綵霞%劉昌奎%譚新穎%囉金超%鬍敏
경채하%류창규%담신영%라금초%호민
干细胞%骨髓干细胞%骨髓间充质干细胞%同种异体骨%下颌骨%修复重建%成骨能力%明胶海绵%骨密度%国家自然科学基金
榦細胞%骨髓榦細胞%骨髓間充質榦細胞%同種異體骨%下頜骨%脩複重建%成骨能力%明膠海綿%骨密度%國傢自然科學基金
간세포%골수간세포%골수간충질간세포%동충이체골%하합골%수복중건%성골능력%명효해면%골밀도%국가자연과학기금
Bone Marrow%Mesenchymal Stem Cell Transplantation%Mandible%Bone Remodeling%Transplantation,Homologous
背景:同种异体骨与自体骨有相似解剖外形和生物学特性,是较佳的生物支架材料。自体骨髓来源的间充质干细胞具有多分化潜能,能向成骨、成软骨细胞分化,加速骨组织及软骨组织的形成。目的:探讨同种异体骨支架复合自体骨髓间充质干细胞促进犬下颌骨半侧缺损的新骨成骨能力。方法:拔出24只比格犬左侧下颌牙,伤口愈合后2个月,人为造成犬下颌骨缺损,对照组用单纯冻干同种异体骨修复,实验组用同种异体冻干骨加自体骨髓间充质干细胞修复。术后4,12,24周对下颌骨体部进行骨密度扫描以及Micro-CT检查。结果与结论:实验组移植后12周开始,下颌骨的骨密度显著高于对照组(P <0.05),随着时间推移,实验组和对照组骨密度均增高,但实验组增高明显高于对照组。随时间推移,实验组骨结构参数成阶梯式递增或递减,对照组虽也有递增或递减,但不明显。术后24周实验组感兴趣区骨小梁分离度大于对照组(P <0.05),骨体积分数、骨小梁数量、骨小梁厚度小于对照组(P<0.05)。结果表明骨髓间充质干细胞能加速同种异体骨的骨改建速度。
揹景:同種異體骨與自體骨有相似解剖外形和生物學特性,是較佳的生物支架材料。自體骨髓來源的間充質榦細胞具有多分化潛能,能嚮成骨、成軟骨細胞分化,加速骨組織及軟骨組織的形成。目的:探討同種異體骨支架複閤自體骨髓間充質榦細胞促進犬下頜骨半側缺損的新骨成骨能力。方法:拔齣24隻比格犬左側下頜牙,傷口愈閤後2箇月,人為造成犬下頜骨缺損,對照組用單純凍榦同種異體骨脩複,實驗組用同種異體凍榦骨加自體骨髓間充質榦細胞脩複。術後4,12,24週對下頜骨體部進行骨密度掃描以及Micro-CT檢查。結果與結論:實驗組移植後12週開始,下頜骨的骨密度顯著高于對照組(P <0.05),隨著時間推移,實驗組和對照組骨密度均增高,但實驗組增高明顯高于對照組。隨時間推移,實驗組骨結構參數成階梯式遞增或遞減,對照組雖也有遞增或遞減,但不明顯。術後24週實驗組感興趣區骨小樑分離度大于對照組(P <0.05),骨體積分數、骨小樑數量、骨小樑厚度小于對照組(P<0.05)。結果錶明骨髓間充質榦細胞能加速同種異體骨的骨改建速度。
배경:동충이체골여자체골유상사해부외형화생물학특성,시교가적생물지가재료。자체골수래원적간충질간세포구유다분화잠능,능향성골、성연골세포분화,가속골조직급연골조직적형성。목적:탐토동충이체골지가복합자체골수간충질간세포촉진견하합골반측결손적신골성골능력。방법:발출24지비격견좌측하합아,상구유합후2개월,인위조성견하합골결손,대조조용단순동간동충이체골수복,실험조용동충이체동간골가자체골수간충질간세포수복。술후4,12,24주대하합골체부진행골밀도소묘이급Micro-CT검사。결과여결론:실험조이식후12주개시,하합골적골밀도현저고우대조조(P <0.05),수착시간추이,실험조화대조조골밀도균증고,단실험조증고명현고우대조조。수시간추이,실험조골결구삼수성계제식체증혹체감,대조조수야유체증혹체감,단불명현。술후24주실험조감흥취구골소량분리도대우대조조(P <0.05),골체적분수、골소량수량、골소량후도소우대조조(P<0.05)。결과표명골수간충질간세포능가속동충이체골적골개건속도。
BACKGROUND:Al ogeneic bone has anatomical appearance and biological features similar to autogenous bone, which is an excel ent biological scaffold material. Mesenchymal stem cel s originating from autogenous bone marrow have mutli-lineage differentiation potential, can differentiate into osteoblasts and chondrocyte, and thus can accelerate the formation of bone tissue and cartilage tissue. OBJECTIVE:To establish the osteogenic ability of al ogeneic bone with autogenous bone marrow mesenchymal stem cel s for repairing major mandibular defects. METHODS:The left mandibular teeth of 24 beagles were extracted, and at 2 months after wound healing, mandibular defects were made artificial y. The beagles were divided into two groups:control group treated with lyophilized al ogeneic bone, and experimental group with autogenous bone marrow mesenchymal stem cel s and lyophilized al ogeneic bone. Densitometry with CT and Micro-CT was conducted 4, 12, and 24 weeks after surgery. RESULTS AND CONCLUSION:Compared with the control group, the bone density of the mandible was significantly higher in the experimental group at 12 weeks after transplantation (P<0.05). Over time, the bone densities in the two groups were both increased, but the bone density in the experimental group was always higher than that in the control group. Bone structure parameters were progressively increased or decreased in the two groups, especial y in the experimental group. At 24 weeks after surgery, the degree of trabecular separation in regions of interest was higher in the experimental group than the control group (P<0.05), but the bone volume fraction, number of trabecular bone, and bone trabecular thickness were significantly lower in the experimental group than the control group (P<0.05). These findings indicate that bone marrow mesenchymal stem cel s are capable of accelerating the reconstruction of al ogeneic bones.