粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2015年
5期
51-54
,共4页
胡二坤%郭兴凤%吴欣欣%崔会娟
鬍二坤%郭興鳳%吳訢訢%崔會娟
호이곤%곽흥봉%오흔흔%최회연
中性蛋白酶%玉米蛋白粉%酶水解%还原力%响应面法
中性蛋白酶%玉米蛋白粉%酶水解%還原力%響應麵法
중성단백매%옥미단백분%매수해%환원력%향응면법
neutral protease%corn protein%enzymatic hydrolysis%reducing power%response surface
采用中性蛋白酶水解玉米蛋白粉,对不同条件下制备的玉米蛋白酶解液的还原力进行了研究,并在此基础上用响应面分析法优化酶水解条件,得到最佳酶解工艺条件为:底物体积分数6.05%,酶解温度55.3℃,加酶量3.88%,pH 7.6。在此条件下,玉米蛋白粉酶解产物的还原力为0.41。试验结果表明,该条件下的酶解产物具有较强的抗氧化能力,在食品工业上有一定的应用价值。
採用中性蛋白酶水解玉米蛋白粉,對不同條件下製備的玉米蛋白酶解液的還原力進行瞭研究,併在此基礎上用響應麵分析法優化酶水解條件,得到最佳酶解工藝條件為:底物體積分數6.05%,酶解溫度55.3℃,加酶量3.88%,pH 7.6。在此條件下,玉米蛋白粉酶解產物的還原力為0.41。試驗結果錶明,該條件下的酶解產物具有較彊的抗氧化能力,在食品工業上有一定的應用價值。
채용중성단백매수해옥미단백분,대불동조건하제비적옥미단백매해액적환원력진행료연구,병재차기출상용향응면분석법우화매수해조건,득도최가매해공예조건위:저물체적분수6.05%,매해온도55.3℃,가매량3.88%,pH 7.6。재차조건하,옥미단백분매해산물적환원력위0.41。시험결과표명,해조건하적매해산물구유교강적항양화능력,재식품공업상유일정적응용개치。
In this paper,neutral protease was used to hydrolyze corn protein,the reducing power of the corn protein hydrolyzate prepared under different conditions was studied,and the enzymatic hydrolysis conditions were optimized by using response surface methodology. The optimum enzymatic hydrolysis conditions were as follows:the substrate concentration 6.05%,enzyme amount 3.88%,hydrolysis temperature 55.3℃,and pH 7.6. The reducing power of the hydrolyzate prepared under the optimum conditions was up to 0.41.The results indicated that the hydrolyzates had excellent antioxidant activity and application value in the food industry.