CAJ | 학술논문

研究从小麦麸皮中提取的阿魏酸糖酯（FGs）对体外蛋白质非酶糖基化反应（NEG）的影响。将牛血清白蛋白和葡萄糖在体外共同孵育，建立蛋白质NEG体外体系；不同浓度的FGs分别与反应体系共孵育，以荧光分光光度法测定FGs对蛋白质非酶糖基化反应产物（AGEs）生成的影响；此外，采用铁氰化钾法检测FGs的总还原能力。结果显示，FGs呈剂量依赖方式抑制AGEs的形成，对蛋白质NEG抑制的IC50为0.0382 mmol/L；铁氰化钾法检测FGs的总还原能力，还原铁离子的IC50为0.0310 mmol/L，低于对照VC的0.0420 mmol/L。FGs抑制蛋白质的非酶糖基化，可能与其强还原能力有关。
연구종소맥부피중제취적아위산당지（FGs）대체외단백질비매당기화반응（NEG）적영향。장우혈청백단백화포도당재체외공동부육，건립단백질NEG체외체계；불동농도적FGs분별여반응체계공부육，이형광분광광도법측정FGs대단백질비매당기화반응산물（AGEs）생성적영향；차외，채용철청화갑법검측FGs적총환원능력。결과현시，FGs정제량의뢰방식억제AGEs적형성，대단백질NEG억제적IC50위0.0382 mmol/L；철청화갑법검측FGs적총환원능력，환원철리자적IC50위0.0310 mmol/L，저우대조VC적0.0420 mmol/L。FGs억제단백질적비매당기화，가능여기강환원능력유관。
The effects of feruloylated glycosides(FGs)extracted from wheat bran on non–enzymatic glycation(NEG)of protein in vitro were studied. Albumin bovine and glucose were used to produce a system for non–enzymatic glycation(NEG)in vitro. Different concentration of FGs and reaction syetem were incubated ,and the effects of FGs on non–enzymatic glycation of protein were tested by fluorescence spectrophotometry. In addition,potassium ferricyanide was used to detect the total reducing powers of FGs. The results showed that FGs could dose–dependently inhibit the formation of AGEs and its IC50 was 0.0382 mmol/L. The result of using potassium ferricyanide to detect the total reducing powers of FGs showed that its IC50 was 0.031 mmol/L,which was lower than the control group that the IC50 of VC was 0.042 mmol/L. FGs had an inhibitory effect on non–enzymatic glycation of protein,which might be related to its strong reducing powers.