中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
18期
2933-2939
,共7页
实验动物%基因病毒载体及相关因子模型%眼%糖尿病视网膜病变大鼠模型%安维汀%房水%细胞因子%血管内皮生长因子%色素上皮衍生因子%结缔组织生长因子%链脲佐菌素
實驗動物%基因病毒載體及相關因子模型%眼%糖尿病視網膜病變大鼠模型%安維汀%房水%細胞因子%血管內皮生長因子%色素上皮衍生因子%結締組織生長因子%鏈脲佐菌素
실험동물%기인병독재체급상관인자모형%안%당뇨병시망막병변대서모형%안유정%방수%세포인자%혈관내피생장인자%색소상피연생인자%결체조직생장인자%련뇨좌균소
Eye%Retinal Diseases%Diabetes Mel itus%Aqueous Humor%Cytokines
背景：关于抑制血管生成药安维汀治疗早期糖尿病视网膜病变大鼠的机制研究多数局限于血管内皮生长因子，而结缔组织生长因子和色素上皮衍生因子也在其中起重要的作用。<br> 目的：探讨安维汀玻璃体腔注射在早期糖尿病视网膜病变模型大鼠应用后房水细胞因子的变化及意义。<br> 方法：经链脲佐菌素诱导10周建立早期糖尿病视网膜病变模型大鼠，分别采用安维汀(1.25，2.5 mg)和生理盐水进行玻璃体腔注射。<br> 结果与结论：ELISA 检测显示，与生理盐水注射组比较，两个安维汀注射组房水中血管内皮生长因子质量浓度降低，色素上皮衍生因子和结缔组织生长因子质量浓度增高(P<0.05)，但两组间上述细胞因子的浓度差异无显著性意义(P>0.05)。结果证实，安维汀玻璃体腔注射在早期糖尿病视网膜病变大鼠应用促进新生血管的细胞因子血管内皮生长因子水平降低，抑制新生血管的细胞因子色素上皮衍生因子质量浓度升高，促进结缔组织生长因子水平升高。
揹景：關于抑製血管生成藥安維汀治療早期糖尿病視網膜病變大鼠的機製研究多數跼限于血管內皮生長因子，而結締組織生長因子和色素上皮衍生因子也在其中起重要的作用。<br> 目的：探討安維汀玻璃體腔註射在早期糖尿病視網膜病變模型大鼠應用後房水細胞因子的變化及意義。<br> 方法：經鏈脲佐菌素誘導10週建立早期糖尿病視網膜病變模型大鼠，分彆採用安維汀(1.25，2.5 mg)和生理鹽水進行玻璃體腔註射。<br> 結果與結論：ELISA 檢測顯示，與生理鹽水註射組比較，兩箇安維汀註射組房水中血管內皮生長因子質量濃度降低，色素上皮衍生因子和結締組織生長因子質量濃度增高(P<0.05)，但兩組間上述細胞因子的濃度差異無顯著性意義(P>0.05)。結果證實，安維汀玻璃體腔註射在早期糖尿病視網膜病變大鼠應用促進新生血管的細胞因子血管內皮生長因子水平降低，抑製新生血管的細胞因子色素上皮衍生因子質量濃度升高，促進結締組織生長因子水平升高。
배경：관우억제혈관생성약안유정치료조기당뇨병시망막병변대서적궤제연구다수국한우혈관내피생장인자，이결체조직생장인자화색소상피연생인자야재기중기중요적작용。<br> 목적：탐토안유정파리체강주사재조기당뇨병시망막병변모형대서응용후방수세포인자적변화급의의。<br> 방법：경련뇨좌균소유도10주건립조기당뇨병시망막병변모형대서，분별채용안유정(1.25，2.5 mg)화생리염수진행파리체강주사。<br> 결과여결론：ELISA 검측현시，여생리염수주사조비교，량개안유정주사조방수중혈관내피생장인자질량농도강저，색소상피연생인자화결체조직생장인자질량농도증고(P<0.05)，단량조간상술세포인자적농도차이무현저성의의(P>0.05)。결과증실，안유정파리체강주사재조기당뇨병시망막병변대서응용촉진신생혈관적세포인자혈관내피생장인자수평강저，억제신생혈관적세포인자색소상피연생인자질량농도승고，촉진결체조직생장인자수평승고。
BACKGROUND:Studies on the inhibitory mechanism of Avastin for early diabetic retinopathy rats are mostly confined to the vascular endothelial growth factor, while connective tissue growth factor and pigment epithelium-derived factor also play an important role in the disease. <br> OBJECTIVE:To evaluate the changed cytokines of aqueous humor and significance of Avastin intravitreous injection in early diabetic retinopathy rats. <br> METHODS:Rat models of early diabetic retinopathy were established by inducing with streptozotocin for 10 weeks. Avastin (1.25 mg and 2.5 mg) and physiological saline were injected in the vitreous space. <br> RESULTS AND CONCLUSION:Enzyme linked immunosorbent assay results revealed that compared with the physiological saline injection group, mass concentration of vascular endothelial growth factor was reduced, mass concentrations of pigment epithelium-derived factor and connective tissue growth factor was increased in aqueous humor of the two Avastin injection groups (P<0.05), but no significant difference in concentration of above cytokines was detected between the two groups (P>0.05). Results verified that Avastin intravitreous injection decreased vascular endothelial growth factor levels, decreased pigment epithelium-derived factor mass concentration and increased connective tissue growth factor levels in rat models of early diabetic retinopathy.
