CAJ | 학술논문

背景：动物实验和临床研究表明在大脑发育的早期进行功能剥夺可能会引起成年期神经和认知功能障碍，但是到目前为止关于嗅觉功能活动是否影响成年豚鼠梨状皮质的神经发生尚无报道。 　　目的：建立嗅觉剥夺模型观察嗅觉经验对成年豚鼠两侧梨状皮质的微管聚合蛋白、小白蛋白、谷氨酸脱羧酶67以及微管相关蛋白与神经元特异性核蛋白共表达的影响。 　　方法：将20只成年豚鼠随机平分成2组，建立嗅觉剥夺模型饲养3周和6周之后灌注取材，应用免疫组织化学技术观察各个时间点豚鼠自身两侧梨状皮质微管相关蛋白、小白蛋白和谷氨酸脱羧酶67的分布，免疫荧光观察微管相关蛋白与神经元特异性核蛋白的共表达。 　　结果与结论：3周和6周组嗅觉未剥夺侧梨状皮质表达的微管相关蛋白、小白蛋白和谷氨酸脱羧酶67阳性细胞数目均明显多于嗅觉剥夺侧梨状皮质，两侧相比差异有显著性意义(P<0.05)；3周组和6周组两侧梨状皮质微管相关蛋白与神经元特异性核蛋白的共表达细胞差异无显著性意义(P>0.05)。提示嗅觉经验对成年豚鼠梨状皮质神经元的成熟及分化起着重要促进作用。
배경：동물실험화림상연구표명재대뇌발육적조기진행공능박탈가능회인기성년기신경화인지공능장애，단시도목전위지관우후각공능활동시부영향성년돈서리상피질적신경발생상무보도。 　　목적：건립후각박탈모형관찰후각경험대성년돈서량측리상피질적미관취합단백、소백단백、곡안산탈최매67이급미관상관단백여신경원특이성핵단백공표체적영향。 　　방법：장20지성년돈서수궤평분성2조，건립후각박탈모형사양3주화6주지후관주취재，응용면역조직화학기술관찰각개시간점돈서자신량측리상피질미관상관단백、소백단백화곡안산탈최매67적분포，면역형광관찰미관상관단백여신경원특이성핵단백적공표체。 　　결과여결론：3주화6주조후각미박탈측리상피질표체적미관상관단백、소백단백화곡안산탈최매67양성세포수목균명현다우후각박탈측리상피질，량측상비차이유현저성의의(P<0.05)；3주조화6주조량측리상피질미관상관단백여신경원특이성핵단백적공표체세포차이무현저성의의(P>0.05)。제시후각경험대성년돈서리상피질신경원적성숙급분화기착중요촉진작용。
BACKGROUND:Animal experiments and clinical studies have shown that, functional deprivation at the early development stage may possibly cause nerve and cognition dysfunction at adulthood. However, no research focuses on the effect of olfactory function on the neurogenesis in piriform cortex of adult guinea pigs. OBJECTIVE:To establish olfactory deprivation models and explore the effect of olfactory experience on the distribution of doublecortin, parvalbumin, glutamic acid decarboxylase 67 and the co-localization of doublecortin with neuron-specific nuclear-binding protein in piriform cortex of adult guinea pigs. METHODS:Twenty guinea pigs were randomly divided into two groups. Olfactory deprivation models were established through unilateral naris-occlusion and the occluded animals were al owed to survive 3 and 6 weeks, then the specimens were harvested. The distribution of doublecortin, parvalbumin, glutamic acid decarboxylase 67 was detected with immunohistochemistry. The co-localization of doublecortin with neuron-specific nuclear-binding protein in piriform cortex of adult guinea pigs was determined with immunofluorescence method. RESULTS AND CONCLUSION:The number of doublecortin, parvalbumin, glutamic acid decarboxylase 67 positive cel s in piriform cortex at the undeprived side was significantly higher than that at the deprived side at 3 and 6 weeks (P<0.05). There was no significant difference in the co-localization of doublecortin with neuron-specific nuclear-binding protein in piriform cortex at 3 weeks group and 6 weeks group (P>0.05). The findings suggest that olfactory experience promotes the maturation and differentiation of neurons in piriform cortex of adult guinea pigs.