中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
18期
2862-2866
,共5页
孙建忠%刘欣伟%管华鹏%张鹏%刘琦%杨珺%郭群峰%倪斌
孫建忠%劉訢偉%管華鵬%張鵬%劉琦%楊珺%郭群峰%倪斌
손건충%류흔위%관화붕%장붕%류기%양군%곽군봉%예빈
实验动物%脑及脊髓损伤模型%人参皂苷Rg1%脊髓损伤%脑源性神经营养因子%转化生长因子β%中枢神经%神经保护
實驗動物%腦及脊髓損傷模型%人參皂苷Rg1%脊髓損傷%腦源性神經營養因子%轉化生長因子β%中樞神經%神經保護
실험동물%뇌급척수손상모형%인삼조감Rg1%척수손상%뇌원성신경영양인자%전화생장인자β%중추신경%신경보호
Ginsenosides%Spinal Cord Injury%Oxidoreductases%Neurturin
背景：转化生长因子β和脑源性神经营养因子是脊髓损伤过程中的主要调节因子，研究转化生长因子β和脑源性神经营养因子在脊髓损伤过程中发生发展机制比较多，对于人参皂甙Rg1干预调节转化生长因子β和脑源性神经营养因子在脊髓损伤发作用的文献鲜有报道。<br> 目的：通过人参皂甙Rg1干预结合转化生长因子β和脑源性神经营养因子在分子蛋白水平上表达量的变化来研究人参皂甙在脊髓损伤后对脊髓及神经的保护作用。<br> 方法：将大鼠随机分为空白对照组、模型组和人参皂苷Rg1干预组。模型组和人参皂苷Rg1干预组大鼠以撞击法建立脊髓损伤模型。人参皂苷Rg1干预组大鼠在造模后24 h以10 mg/kg的人参皂甙Rg1腹腔注射，1次/d，连续14 d。空白对照组和模型组大鼠注射等体积生理盐水。<br> 结果与结论：与空白对照组相比，模型组和人参皂苷Rg1干预组大鼠血清中的丙二醛含量升高，超氧化物歧化酶的含量降低，脊髓组织中转化生长因子β表达水平增加，脑源性神经营养因子表达水平降低；与模型组相比，人参皂苷Rg1干预组大鼠血清中的丙二醛降低，超氧化物歧化酶的含量增加，脊髓组织中转化生长因子β表达水平降低，脑源性神经营养因子表达水平升高。提示人参皂苷Rg1对损伤的大鼠脊髓组织具有保护作用。
揹景：轉化生長因子β和腦源性神經營養因子是脊髓損傷過程中的主要調節因子，研究轉化生長因子β和腦源性神經營養因子在脊髓損傷過程中髮生髮展機製比較多，對于人參皂甙Rg1榦預調節轉化生長因子β和腦源性神經營養因子在脊髓損傷髮作用的文獻鮮有報道。<br> 目的：通過人參皂甙Rg1榦預結閤轉化生長因子β和腦源性神經營養因子在分子蛋白水平上錶達量的變化來研究人參皂甙在脊髓損傷後對脊髓及神經的保護作用。<br> 方法：將大鼠隨機分為空白對照組、模型組和人參皂苷Rg1榦預組。模型組和人參皂苷Rg1榦預組大鼠以撞擊法建立脊髓損傷模型。人參皂苷Rg1榦預組大鼠在造模後24 h以10 mg/kg的人參皂甙Rg1腹腔註射，1次/d，連續14 d。空白對照組和模型組大鼠註射等體積生理鹽水。<br> 結果與結論：與空白對照組相比，模型組和人參皂苷Rg1榦預組大鼠血清中的丙二醛含量升高，超氧化物歧化酶的含量降低，脊髓組織中轉化生長因子β錶達水平增加，腦源性神經營養因子錶達水平降低；與模型組相比，人參皂苷Rg1榦預組大鼠血清中的丙二醛降低，超氧化物歧化酶的含量增加，脊髓組織中轉化生長因子β錶達水平降低，腦源性神經營養因子錶達水平升高。提示人參皂苷Rg1對損傷的大鼠脊髓組織具有保護作用。
배경：전화생장인자β화뇌원성신경영양인자시척수손상과정중적주요조절인자，연구전화생장인자β화뇌원성신경영양인자재척수손상과정중발생발전궤제비교다，대우인삼조대Rg1간예조절전화생장인자β화뇌원성신경영양인자재척수손상발작용적문헌선유보도。<br> 목적：통과인삼조대Rg1간예결합전화생장인자β화뇌원성신경영양인자재분자단백수평상표체량적변화래연구인삼조대재척수손상후대척수급신경적보호작용。<br> 방법：장대서수궤분위공백대조조、모형조화인삼조감Rg1간예조。모형조화인삼조감Rg1간예조대서이당격법건립척수손상모형。인삼조감Rg1간예조대서재조모후24 h이10 mg/kg적인삼조대Rg1복강주사，1차/d，련속14 d。공백대조조화모형조대서주사등체적생리염수。<br> 결과여결론：여공백대조조상비，모형조화인삼조감Rg1간예조대서혈청중적병이철함량승고，초양화물기화매적함량강저，척수조직중전화생장인자β표체수평증가，뇌원성신경영양인자표체수평강저；여모형조상비，인삼조감Rg1간예조대서혈청중적병이철강저，초양화물기화매적함량증가，척수조직중전화생장인자β표체수평강저，뇌원성신경영양인자표체수평승고。제시인삼조감Rg1대손상적대서척수조직구유보호작용。
BACKGROUND:Transformation growth factor-β(TGF-β) and brain-derived neurotrophic factor (BDNF) are the main regulatory factors in the process of spinal cord injury. There are many researches for TGF-βand BDNF pathogenesis in the spinal cord injury, but the regulation of Ginsenoside Rg1 intervention on TGF-βand BDNF in the spinal cord injury is rarely reported. <br> OBJECTIVE:To observe the effect of Ginsenoside Rg1 intervention on TGF-βand BDNF expression at themolecular protein levels, and to study the protection effect of Ginsenoside Rg1 on the spinal cord and nerve function after spinal cord injury. <br> METHODS:Experimental rats were randomly divided into blank control group, model group and Ginsenoside Rg1 group. In the model and Ginsenoside Rg1 groups, spinal cord injury model was established with the impact method in rats. In the Ginsenoside Rg1 group, rats were intraperitoneal y injected with 10 mg/kg Ginsenoside Rg1 24 hours after modeling, once per day, for 14 days. Rats in the blank control and model groups were injected with equal saline. <br> RESULTS AND CONCLUSION:Compared with the control group, serum malondialdehyde levels increased, the content of superoxide dismutase decreased, TGF-βexpression levels in spinal cord tissue increased, and BDNF expression levels decreased in the model and Ginsenoside Rg1 groups. Compared with the model group, serum malondialdehyde levels decreased, the content of superoxide dismutase increased, TGF-βexpression levels in spinal cord tissue decreased, and BDNF expression levels increased in the Ginsenoside Rg1 group. Ginsenoside Rg1 can protect the injury spinal cord in rats after spinal cord injury.
