CAJ | 학술논문

背景：印第安刺猬蛋白(Ihh)及其信号蛋白Gli1以及Runt相关转录因子2(Runx2)是骨关节炎密切相关的因子，在骨关节炎发病过程中，是引起关节退行性改变的重要原因之一。 　　目的：探索Ihh，Gli1以及Runx2在骨关节炎发病过程中的作用。 　　方法：将30只SD大鼠随机分成对照组(n=10)和模型组(n=20)。模型组大鼠取一侧膝关节行前交叉韧带离断建立骨关节炎模型，分别于造模后4周及12周，各取10只大鼠进行观察。对照组大鼠仅暴露前交叉韧带，于术后12周进行观察。 　　结果与结论：与对照组相比，造模4周后可见骨关节炎模型大鼠膝关节出现软骨退变，膝关节软骨中 Ihh， Gli1及Runx2表达水平明显增高，而造模12周后软骨退变进一步加重，但膝关节软骨中Ihh，Gli1及Runx2表达水平下降。提示Ihh，Gli1及Runx2在骨关节炎退变过程中起重要作用，其表达水平在骨关节炎的早期即明显升高，可作为骨关节炎防治研究中的评价指标。
배경：인제안자위단백(Ihh)급기신호단백Gli1이급Runt상관전록인자2(Runx2)시골관절염밀절상관적인자，재골관절염발병과정중，시인기관절퇴행성개변적중요원인지일。 　　목적：탐색Ihh，Gli1이급Runx2재골관절염발병과정중적작용。 　　방법：장30지SD대서수궤분성대조조(n=10)화모형조(n=20)。모형조대서취일측슬관절행전교차인대리단건립골관절염모형，분별우조모후4주급12주，각취10지대서진행관찰。대조조대서부폭로전교차인대，우술후12주진행관찰。 　　결과여결론：여대조조상비，조모4주후가견골관절염모형대서슬관절출현연골퇴변，슬관절연골중 Ihh， Gli1급Runx2표체수평명현증고，이조모12주후연골퇴변진일보가중，단슬관절연골중Ihh，Gli1급Runx2표체수평하강。제시Ihh，Gli1급Runx2재골관절염퇴변과정중기중요작용，기표체수평재골관절염적조기즉명현승고，가작위골관절염방치연구중적평개지표。
BACKGROUND:Indian hedgehog homolog (Ihh) protein and its signal protein Gli1, as wel as Runt related transcription factor 2 (Runx2) are closely related to the pathogenesis of osteoarthritis. The increased expression of these factors is one of the major causes of degenerative joint changes. OBJECTIVE:To explore the roles of Ihh, Gli1 and Runx2 in the development of osteoarthritis. METHODS:Thirty Sprague-Dawley rats were randomly divided into two groups:control group (n=10) and model group (n=20). In the model group, rats received unilateral anterior cruciate ligament transection, to establish osteoarthritis model. Ten experimental rats were kil ed at 4 and 12 weeks after surgery respectively. Another 10 rats received unilateral knee arthrotomy as pseudo-operation controls and 10 pseudo-operation rats were kil ed at 12 weeks after surgery. RESULTS AND CONCLUSION:In the model group, cartilage degeneration was obvious at 4 weeks and became severer at 12 weeks after anterior cruciate ligament transection operation. Expression levels of Ihh, Gli1 and Runx2 in the cartilage were increased significantly at 4 weeks after operation, but decreased at 12 weeks after operation. Experimental findings indicate that Ihh, Gli1 and Runx2 play important roles in the development of osteoarthritis, and their expression levels are significantly increased at early stage of osteoarthritis, which can be regarded as the indicators in the prophylaxis and treatment research of osteoarthritis.