CAJ | 학술논문

实验动物%骨及关节损伤模型%髓样细胞诱发受体2%类风湿关节炎%胶原性关节炎%滑膜组织%炎症%肿瘤坏死因子α%白细胞介素1β%白细胞介素10%大鼠%广东省自然科学基金實驗動物%骨及關節損傷模型%髓樣細胞誘髮受體2%類風濕關節炎%膠原性關節炎%滑膜組織%炎癥%腫瘤壞死因子α%白細胞介素1β%白細胞介素10%大鼠%廣東省自然科學基金
실험동물%골급관절손상모형%수양세포유발수체2%류풍습관절염%효원성관절염%활막조직%염증%종류배사인자α%백세포개소1β%백세포개소10%대서%광동성자연과학기금
Tissue Engineering%Arthritis,Rheumatoid%Arthritis,Experimental%Synovial Membrane%Cytokines

背景：髓样细胞诱发受体2在类风湿关节炎活动期患者滑膜组织呈高表达，但其在类风湿关节炎发病机制中发挥的作用，目前并不清楚。 　　目的：观察髓样细胞诱发受体2在牛源性Ⅱ型胶原诱导的关节炎大鼠模型滑膜组织中的表达。 　　方法：制备胶原诱导关节炎模型大鼠，动态观察大鼠各项关节炎的活动指标和滑膜组织的病理学改变，RT-PCR法检测滑膜组织中髓样细胞诱发受体2、肿瘤坏死因子α、白细胞介素1β、白细胞介素10 mRNA的表达， Western blot及免疫组织化学法分别检测关节滑膜组织中髓样细胞诱发受体2的蛋白表达及定位。 　　结果与结论：模型组大鼠免疫13 d后出现足爪红肿，关节炎指数评分逐渐升高(P <0.01)，炎症高峰期在19-25 d，苏木精-伊红染色可见诱导性关节炎大鼠滑膜组织增生及炎性细胞浸润、软骨骨质破坏。与对照组相比，模型组大鼠关节滑膜髓样细胞诱发受体2的mRNA和蛋白的表达、肿瘤坏死因子α、白细胞介素1βmRNA表达均明显升高(P<0.05或P<0.01)，白细胞介素10 mRNA表达显著降低(P<0.05)。结果证实，髓样细胞诱发受体2可能是类风湿关节炎模型大鼠发病过程中一个重要的炎症调节递质，其反应性升高在类风湿关节炎模型大鼠发病中发挥着重要作用。
배경：수양세포유발수체2재류풍습관절염활동기환자활막조직정고표체，단기재류풍습관절염발병궤제중발휘적작용，목전병불청초。 　　목적：관찰수양세포유발수체2재우원성Ⅱ형효원유도적관절염대서모형활막조직중적표체。 　　방법：제비효원유도관절염모형대서，동태관찰대서각항관절염적활동지표화활막조직적병이학개변，RT-PCR법검측활막조직중수양세포유발수체2、종류배사인자α、백세포개소1β、백세포개소10 mRNA적표체， Western blot급면역조직화학법분별검측관절활막조직중수양세포유발수체2적단백표체급정위。 　　결과여결론：모형조대서면역13 d후출현족조홍종，관절염지수평분축점승고(P <0.01)，염증고봉기재19-25 d，소목정-이홍염색가견유도성관절염대서활막조직증생급염성세포침윤、연골골질파배。여대조조상비，모형조대서관절활막수양세포유발수체2적mRNA화단백적표체、종류배사인자α、백세포개소1βmRNA표체균명현승고(P<0.05혹P<0.01)，백세포개소10 mRNA표체현저강저(P<0.05)。결과증실，수양세포유발수체2가능시류풍습관절염모형대서발병과정중일개중요적염증조절체질，기반응성승고재류풍습관절염모형대서발병중발휘착중요작용。
BACKGROUND:Triggering receptor expressed on myeloid cel s 2 (TREM-2) is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis stil remains unclear. OBJECTIVE:To explore the TREM-2 expression in the synovial tissue of col agen type II-induced arthritis rats. METHODS:The col agen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamical y observed. The mRNA levels of TREM-2, tumor necrosis factor-α, interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively. RESULTS AND CONCLUSION:At day 13 after immunization, the paws of model rats appeared red and swel ing, the arthritis index scores were increased (P<0.01). At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of col agen-induced arthritis rats were proliferated and were infiltrated by inflammatory cel s, cartilage was destroyed. Compared with the control group, the expression of TREM-2 mRNA and protein, the mRNA levels of tumor necrosis factor-αand interleukin-1βin synovial tissue of the model rats were significantly increased (P<0.05 or P<0.01), while interleukin-10 mRNA expression was significantly decreased (P<0.05). Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of col agen-induced arthritis.