中国肝脏病杂志（电子版）
中國肝髒病雜誌（電子版）
중국간장병잡지（전자판）
CHINESE JOURNAL OF LIVER DISEASES(ELECTRONIC VERSION)
2015年
1期
10-14
,共5页
张韬%程变巧%林伟国%朱琪
張韜%程變巧%林偉國%硃琪
장도%정변교%림위국%주기
慢病毒属%大鼠%转染
慢病毒屬%大鼠%轉染
만병독속%대서%전염
Lentivirus%Rats%Transfection
目的：探讨PXR对CCl4诱导的大鼠肝损伤的保护作用。方法雄性SD大鼠48只，随机分为4组，空白组不做任何处理；模型组通过CCl4灌胃诱导肝损伤；空载体组在建立肝损伤模型前3天静脉注射慢病毒空载体；PXR慢病毒组在建立肝损伤模型前3天静脉注射PXR重组慢病毒。通过血清AST和ALT检测和肝脏HE染色评估肝损伤程度；Western blot及RT-PCR检测大鼠肝组织中PXR、NF-κB、IL-1、IL-2和TNF-α的表达。结果慢病毒介导PXR体内转染后肝损伤大鼠血清AST和ALT明显降低，肝损伤及纤维化程度明显减轻，PXR表达水平明显升高，相反，NF-κB、IL-1、IL-2和TNF-α的表达水平明显降低。结论 PXR可能通过抑制NF-κB的表达，下调炎症相关因子IL-1、IL-2和TNF-α的表达，从而对CCl4诱导的大鼠肝损伤具有一定的保护作用，可为肝损伤的治疗提供一种新的途径和思路。
目的：探討PXR對CCl4誘導的大鼠肝損傷的保護作用。方法雄性SD大鼠48隻，隨機分為4組，空白組不做任何處理；模型組通過CCl4灌胃誘導肝損傷；空載體組在建立肝損傷模型前3天靜脈註射慢病毒空載體；PXR慢病毒組在建立肝損傷模型前3天靜脈註射PXR重組慢病毒。通過血清AST和ALT檢測和肝髒HE染色評估肝損傷程度；Western blot及RT-PCR檢測大鼠肝組織中PXR、NF-κB、IL-1、IL-2和TNF-α的錶達。結果慢病毒介導PXR體內轉染後肝損傷大鼠血清AST和ALT明顯降低，肝損傷及纖維化程度明顯減輕，PXR錶達水平明顯升高，相反，NF-κB、IL-1、IL-2和TNF-α的錶達水平明顯降低。結論 PXR可能通過抑製NF-κB的錶達，下調炎癥相關因子IL-1、IL-2和TNF-α的錶達，從而對CCl4誘導的大鼠肝損傷具有一定的保護作用，可為肝損傷的治療提供一種新的途徑和思路。
목적：탐토PXR대CCl4유도적대서간손상적보호작용。방법웅성SD대서48지，수궤분위4조，공백조불주임하처리；모형조통과CCl4관위유도간손상；공재체조재건립간손상모형전3천정맥주사만병독공재체；PXR만병독조재건립간손상모형전3천정맥주사PXR중조만병독。통과혈청AST화ALT검측화간장HE염색평고간손상정도；Western blot급RT-PCR검측대서간조직중PXR、NF-κB、IL-1、IL-2화TNF-α적표체。결과만병독개도PXR체내전염후간손상대서혈청AST화ALT명현강저，간손상급섬유화정도명현감경，PXR표체수평명현승고，상반，NF-κB、IL-1、IL-2화TNF-α적표체수평명현강저。결론 PXR가능통과억제NF-κB적표체，하조염증상관인자IL-1、IL-2화TNF-α적표체，종이대CCl4유도적대서간손상구유일정적보호작용，가위간손상적치료제공일충신적도경화사로。
Objective To investigate the protective effect of PXR in CCl4-induced liver injury in rat.Methods Forty eight Sprague-Dawley male rats were randomly divided into four groups: control group (without treatment); model group, which was treated with CCl4 to induce liver injury; empty vector group, which was intravenously injected with lentiviral vector three days before liver injury; PXR recombinant lentivirus group was intravenously injected with PXR recombinant lentivirus three days before liver injury. The degree of liver injury was evaluated by HE staining and levels of serum AST and ALT. The expression of PXR, NF-κB, IL-1, IL-2 and TNF-α in liver were assayed by RT-PCR and Western blot.Results After intravenous injection with PXR recombinant lentivirus, ifbrosis and serum AST and ALT in rat with liver injury reduced signiifcantly. The expression of PXR in liver increased signiifcantly. On the contrary, the expression of NF-κB, IL-1, IL-2 and TNF-α in liver decreased signiifcantly.Conclusions PXR plays a protective role in CCl4-induced liver injury in rat. It may through inhibit the expression of NF-κB, further to down-regulate the expression of IL-1, IL-2 and TNF-α, which provides a new way for treatment of liver injury.
