CAJ | 학술논문

目的：检测miR-339-5p在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma, DLBCL)中的表达,探讨miR-339-5p表达与DLBCL临床病理特征的关系。方法采用显色原位杂交技术检测123例 DLBCL 和20例淋巴结反应性增生( reactive lymphoid hyperplasia, RH)组织中miR-339-5p的表达,并采用免疫组化EnVision两步法检测DLBCL组织中Ki-67和BCL-6蛋白的表达,分析miR-339-5p与BCL-6表达的相关性及二者表达与DLBCL临床病理特征的关系。结果 DLBCL组织中miR-339-5p的阳性率(39．8%,49/123)显著低于RH组织(90．0%,18/20)。活化的B细胞型(ABC型)DLBCL组织中miR-339-5p阳性率(31．0%,22/71)明显低于生发中心的次级B细胞型(GCB型)(51．9%,27/52)。 miR-339-5p在DLBCL中表达降低与Ann Arbor分期晚以及国际预后指数IPI评分高有关(P均<0．05)。 ABC型、GCB型DLBCL中miR-339-5p阴性患者生存率均明显低于miR-339-5p阳性患者(P均<0．01)。 DLBCL中miR-339-5p与BCL-6蛋白表达呈显著负相关(P<0．01)。结论miR-339-5p低表达可能与DLBCL进展和预后不良相关。
목적：검측miR-339-5p재미만대B세포림파류(diffuse large B-cell lymphoma, DLBCL)중적표체,탐토miR-339-5p표체여DLBCL림상병리특정적관계。방법채용현색원위잡교기술검측123례 DLBCL 화20례림파결반응성증생( reactive lymphoid hyperplasia, RH)조직중miR-339-5p적표체,병채용면역조화EnVision량보법검측DLBCL조직중Ki-67화BCL-6단백적표체,분석miR-339-5p여BCL-6표체적상관성급이자표체여DLBCL림상병리특정적관계。결과 DLBCL조직중miR-339-5p적양성솔(39．8%,49/123)현저저우RH조직(90．0%,18/20)。활화적B세포형(ABC형)DLBCL조직중miR-339-5p양성솔(31．0%,22/71)명현저우생발중심적차급B세포형(GCB형)(51．9%,27/52)。 miR-339-5p재DLBCL중표체강저여Ann Arbor분기만이급국제예후지수IPI평분고유관(P균<0．05)。 ABC형、GCB형DLBCL중miR-339-5p음성환자생존솔균명현저우miR-339-5p양성환자(P균<0．01)。 DLBCL중miR-339-5p여BCL-6단백표체정현저부상관(P<0．01)。결론miR-339-5p저표체가능여DLBCL진전화예후불량상관。
Purpose To investigate the relevance between the expression of miR-339-5p and the clinicopathological characteristics in diffuse large B-cell lymphoma (DLBCL). Methods The level of miR-339-5p expression was detected in 123 cases of diffuse large B-cell lymphoma tissues and 20 cases of reactive lymphoid hyperplasia tissues by chromogenic in situ hybridization ( CISH) technique. The expression of Ki-67 and BCL-6 protein was examined in diffuse large B-cell lymphoma tissues by immunohistochemical technique (IHC) (EnVision two-steps), and the correlation between the expression of miR-339-5p and BCL-6 and the clinicopathological param-eters was also analyzed. Results The positive rates of miR-339-5p were 39. 8% (49/123) in DLBCL tissues, which was significantly lower than that in RH tissues (90%, 18/20). The positive rates of miR-339-5p were 31. 0% (22/71) for germinal center B-cell-like (ABC type) DLBCL, which was significantly lower than that in activated B-cell-like (GCB type) DLBCL (27/52, 51. 9%). The low-er expression of miR-339-5p in DLBCL was correlated with late Ann Arbor staging and high-risk group of international prognostic index (P<0. 05). The survival rates of miR-339-5p negative patients of ABC type and GCB type of DLBCL were significantly lower than that of the positive patients (P<0. 01). The levels of miR-339-5p expression in DLBCL were negatively correlated with the levels of BCL-6 expression in DLBCL (P<0. 01). Conclusion The low expression of miR-339-5p might be relatived with the progression and poor prognosis of DLBCL.