CAJ | 학술논문

目的：探讨Plk1(polo-like kinase1)和Cyclin B1、p21WAF1在子宫颈癌中的表达及其与临床病理因素之间的关系。方法利用组织芯片技术,结合免疫组化EliVision 法对102例子宫颈癌、20例子宫颈上皮内瘤变(cervical intraepithelial neoplasia, CIN)、20例正常子宫颈组织中Plk1和Cyclin B1、p21WAF1的表达进行检测,并分析相关数据。结果 Plk1在子宫颈癌、CIN 中的阳性率分别为70．5%、55．0%,明显高于正常子宫颈组织(10%),差异有统计学意义(P <0．01)。Cyclin B1在子宫颈癌、 CIN 中的阳性率分别为52．9%,30．0%,明显高于正常子宫颈组织(10．0%),差异有统计学意义(P <0．01)。p21WAF1在子宫颈癌、CIN 中的阳性率分别为23．5%、10．0%,明显高于正常子宫颈组织(0),差异有统计学意义(P <0．05)。Plk1、Cyclin B1和 p21WAF1在子宫颈癌、CIN 中的表达差异均无统计学意义(P >0．05)。Plk1表达与子宫颈癌间质浸润深度相关(P <0．05)。Cyclin B1表达与子宫颈癌间质浸润深度及淋巴结转移相关(P <0．05)。p21WAF1在子宫颈癌中的表达与组织学分级相关(P <0．01)。组织学分级低的子宫颈癌中p21WAF1阳性率高。Plk1和Cyclin B1在子宫颈癌中的表达呈正相关(rs =0．297,P =0．002)。Plk1和p21WAF1在子宫颈癌中的表达呈负相关(rs =-0．403,P <0．001)。结论 Plk1、Cyclin B1和p21WAF1的表达与子宫颈癌的发生、发展相关,且前两者与子宫颈癌预后相关,三者联合检测有可能成为子宫颈癌临床治疗的新靶点。
목적：탐토Plk1(polo-like kinase1)화Cyclin B1、p21WAF1재자궁경암중적표체급기여림상병리인소지간적관계。방법이용조직심편기술,결합면역조화EliVision 법대102례자궁경암、20례자궁경상피내류변(cervical intraepithelial neoplasia, CIN)、20례정상자궁경조직중Plk1화Cyclin B1、p21WAF1적표체진행검측,병분석상관수거。결과 Plk1재자궁경암、CIN 중적양성솔분별위70．5%、55．0%,명현고우정상자궁경조직(10%),차이유통계학의의(P <0．01)。Cyclin B1재자궁경암、 CIN 중적양성솔분별위52．9%,30．0%,명현고우정상자궁경조직(10．0%),차이유통계학의의(P <0．01)。p21WAF1재자궁경암、CIN 중적양성솔분별위23．5%、10．0%,명현고우정상자궁경조직(0),차이유통계학의의(P <0．05)。Plk1、Cyclin B1화 p21WAF1재자궁경암、CIN 중적표체차이균무통계학의의(P >0．05)。Plk1표체여자궁경암간질침윤심도상관(P <0．05)。Cyclin B1표체여자궁경암간질침윤심도급림파결전이상관(P <0．05)。p21WAF1재자궁경암중적표체여조직학분급상관(P <0．01)。조직학분급저적자궁경암중p21WAF1양성솔고。Plk1화Cyclin B1재자궁경암중적표체정정상관(rs =0．297,P =0．002)。Plk1화p21WAF1재자궁경암중적표체정부상관(rs =-0．403,P <0．001)。결론 Plk1、Cyclin B1화p21WAF1적표체여자궁경암적발생、발전상관,차전량자여자궁경암예후상관,삼자연합검측유가능성위자궁경암림상치료적신파점。
Purpose To investigate the expression of polo-like kinase 1 (Plk1) and Cyclin B1, p21WAF1in cervical carcinoma, and to determine the relationship between the expression of the three proteins and tumor clinicopathological features. Methods The expres-sion of Plk1, Cyclin B1 and p21WAF1 was detected in 102 cases of cervical carcinoma, 20 cases of (cervical intraepithelial neoplasia, CIN) , and 20 cases of nomal cervical tissues by the technique of tissue chip and immunohistochemical staining of EliVision. Statistical analyses of the data were performed with SPSS 19. 0 software. Results The positive rates of Plk1 in cervical carcinoma and CIN were 70. 5%, 55. 0%, respectively, which were significantly higher than normal cervical tissues (10%) (P<0. 01);The positive rates of Cyclin B1 in cervical carcinoma and CIN were 52. 9% and 30. 0%, respectively, which were significantly higher than normal cervical tissues (10%)(P <0.01); The positive rates of p21WAF1 in cervical carcinoma and CIN were 23.5% and 10.0%, respectively, which were significantly higher than normal cervical tissues ( 0 ) ( P<0. 01 ) . There were no significant differences between cervical carcinoma and CIN in the positive rates of Plk1, Cyclin B1 and p21WAF1. The expression of Plk1 was associated with the depth of carci-noma invasion (P<0. 05), that of Cyclin B1 was associated with lymph node metastases and the depth of carcinoma invasion (P<0. 05)and that of p21WAF1 in cervical carcinoma was associated with histological grade (P<0. 05). In cervical carcinoma, the expres-sion of Plk1 was positively correlated with Cyclin B1 (rs =0. 297, P=0. 002) and negatively correlated with p21WAF1(rs = -0. 403, P<0. 001). Conclusion The expression of Plk1, Cyclin B1 and p21WAF1 is involved in the occurrence and development of cervical carcinoma, and the former two are also related with prognosis of cervical carcinoma. The combination of the three would provide a new target for clinical treatment.