CAJ | 학술논문

目的：检测NKX2．2蛋白在胃肠胰食管神经内分泌肿瘤中的表达,探讨其表达与临床病理特征的关系。方法采用免疫组化法检测41例胃肠胰食管神经内分泌肿瘤中NKX2．2、Syn及CgA的表达,并复习相关文献。结果 NKX2．2蛋白阳性定位于细胞核,在7个原发部位的神经内分泌肿瘤中均有不同程度的表达,在4例正常胰腺胰岛细胞中弥漫强阳性表达, NKX2．2、Syn及CgA在小肠、直肠及胰腺神经内分泌肿瘤中的总阳性率分别为100%、100%、46．7%；NKX2．2在前肠、中后肠的阳性率分别为30%和87%,差异有统计学意义(χ2=11．09,P=0．001)。 NKX2．2蛋白表达与患者性别、年龄、分级、肿瘤最大径及淋巴结转移无明显相关性。结论 NKX2．2作为一种新型的神经内分泌标志物,在诊断小肠、直肠及胰腺神经内分泌肿瘤上明显优于CgA,联合检测NKX2．2、Syn、CgA可提高小肠、直肠及胰腺神经内分泌肿瘤诊断的准确性。
목적：검측NKX2．2단백재위장이식관신경내분비종류중적표체,탐토기표체여림상병리특정적관계。방법채용면역조화법검측41례위장이식관신경내분비종류중NKX2．2、Syn급CgA적표체,병복습상관문헌。결과 NKX2．2단백양성정위우세포핵,재7개원발부위적신경내분비종류중균유불동정도적표체,재4례정상이선이도세포중미만강양성표체, NKX2．2、Syn급CgA재소장、직장급이선신경내분비종류중적총양성솔분별위100%、100%、46．7%；NKX2．2재전장、중후장적양성솔분별위30%화87%,차이유통계학의의(χ2=11．09,P=0．001)。 NKX2．2단백표체여환자성별、년령、분급、종류최대경급림파결전이무명현상관성。결론 NKX2．2작위일충신형적신경내분비표지물,재진단소장、직장급이선신경내분비종류상명현우우CgA,연합검측NKX2．2、Syn、CgA가제고소장、직장급이선신경내분비종류진단적준학성。
Purpose To detect the expression of NKX2. 2 protein in gastrointestinal, pancreatic and esophageal neuroendocrine tumors and the correlation between the expression of NKX2. 2 and the clinicopathologic parameters. Methods 41 cases of gastrointestinal, pancreatic and esophageal neuroendocrine tumors were collected. The expression of NKX2. 2, Syn and CgA in neuroendocrine tumor samples were checked by using immunohistochemical staining. Results The positive expression of NKX2. 2 protein was localized in the nucleus. NKX2. 2 protein showed positive staining in neuroendocrine tumors of the seven original sites. In the four cases of normal pancreatic islet cells also showed strongly diffuse positive nuclear staining. The positive rates of NKX2. 2, Syn and CgA protein in the small intestine, rectum, pancreatic neuroendocrine tumors were 100%, 100% and 46. 7%, respectively. The positive rates of NKX2. 2 in foregut, midgut and hindgut were 30% and 87% (χ2 = 11. 09, P=0. 001). Positive NKX2. 2 protein expression was not associated with gender, age group, grade, tumor size and lymph node metastasis. Conclusion NKX2. 2, as a new type of neuroendo-crine markers, is obviously superior to CgA in the diagnosis of neuroendocrine tumors in the small bowel, rectum and pancreas. NKX2. 2, Syn and CgA, a panel approach may be beneficial to enhance diagnostic accuracy of the neuroendocrine tumors.