CAJ | 학술논문

背景：目前治疗类风湿关节炎主要的药物有慢作用抗风湿药、生物制剂及植物药物，植物药因价格便宜、不良反应小而越来越受到瞩目。 　　目的：观察胶原诱导性关节炎模型大鼠滑膜中肿瘤坏死因子α、白细胞介素1β表达及白喉乌头对其表达的影响。 　　方法：将45只大鼠随机分为正常对照组8只、造模组37只，造模组于大鼠足跖及尾根部多点皮内注射牛Ⅱ型胶原乳剂建立胶原诱导性关节炎大鼠模型。造模成功后，随机选取24只造模成功大鼠进行后续实验，将其随机分为模型组、雷公藤组和白喉乌头组，每组各8只，每周对3个造模后干预组和正常对照组共4组大鼠进行关节炎评分。经过灌胃给药治疗4周后，通过免疫组织化学染色检测肿瘤坏死因子α、白细胞介素1β在滑膜中的表达。 　　结果与结论：与模型组比较，治疗后白喉乌头组、雷公藤组关节炎评分明显降低(P<0.05)。肿瘤坏死因子α、白细胞介素1β在模型组滑膜中表达明显高于正常对照组(P <0.05)，在白喉乌头组、雷公藤组中表达低于模型组(P<0.05)。结果证实，白喉乌头治疗类风湿关节炎的机制可能与其抑制肿瘤坏死因子α和白细胞介素1β表达有关。
배경：목전치료류풍습관절염주요적약물유만작용항풍습약、생물제제급식물약물，식물약인개격편의、불량반응소이월래월수도촉목。 　　목적：관찰효원유도성관절염모형대서활막중종류배사인자α、백세포개소1β표체급백후오두대기표체적영향。 　　방법：장45지대서수궤분위정상대조조8지、조모조37지，조모조우대서족척급미근부다점피내주사우Ⅱ형효원유제건립효원유도성관절염대서모형。조모성공후，수궤선취24지조모성공대서진행후속실험，장기수궤분위모형조、뢰공등조화백후오두조，매조각8지，매주대3개조모후간예조화정상대조조공4조대서진행관절염평분。경과관위급약치료4주후，통과면역조직화학염색검측종류배사인자α、백세포개소1β재활막중적표체。 　　결과여결론：여모형조비교，치료후백후오두조、뢰공등조관절염평분명현강저(P<0.05)。종류배사인자α、백세포개소1β재모형조활막중표체명현고우정상대조조(P <0.05)，재백후오두조、뢰공등조중표체저우모형조(P<0.05)。결과증실，백후오두치료류풍습관절염적궤제가능여기억제종류배사인자α화백세포개소1β표체유관。
BACKGROUND:The majority of rheumatoid arthritis treatment is chronic anti-arthritis drugs, biological agents and plant drugs. Among them, plant drugs have been widely concerned due to low cost and few adverse effects. OBJECTIVE:To observe the expression of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in the synovium of col agen-induced arthritis rats, and explore the effect of Aconitum leucostomum Worosch. on the expression. METHODS:Forty-five rats were randomly divided into normal control group (8 rats) and model group (37 rats). The col agen-induced arthritis model was established with the injection of type II bovine col agen into the end of the tail and paws. After the success of modeling, the 24 successful model rats were randomly selected and divided into model group (8 rats), Tripterygium wolfprdi polyglycoside group (8 rats) and Aconitum leucostomum Worosch. group (8 rats). The arthritis index of the rats in the three intervention groups and one control group were evaluated weekly. After treated by intragastric administration for 4 weeks (Tripterygium wolfprdi polyglycoside group and Aconitum leucostomum Worosch. group were taken by the corresponding drug solution, model group and normal control group were taken by the same volume of physiological saline), the expressions of TNF-αand IL-1βin the synovium were tested by immunohistochemistry. RESULTS AND CONCLUSION:Compared with the model group, the arthritis index of mice in Tripterygium wolfprdi polyglycoside group and Aconitum leucostomum Worosch. group was decreased significantly after treatment (P<0.05). The expression levels of TNF-αand IL-1βin the synovium of model group were significantly higher than those of the normal control group (P<0.05). After treatment with Aconitum leucostomum Worosch. and Tripterygium wolfprdi polyglycoside, the expression levels of TNF-αand IL-1βin the synovium was decreased compared with the model group (P<0.05). Experimental findings indicated that, the mechanism that Aconitum leucostomum Worosch. treats rheumatoid arthritis is related to the inhibition of TNF-αand IL-1β.