CAJ | 학술논문

背景：木瓜蛋白酶诱导大鼠膝关节骨性关节炎是常用造模方法，能获得稳定的骨关节炎模型。 　　目的：观察木瓜蛋白酶诱导大鼠膝早期骨关节炎进程中透射电镜下关节软骨细胞超微结构的变化规律。 　　方法：将18只SD大鼠随机分为3组，2只为正常对照组不做干预；16只大鼠右膝关节腔注射木瓜蛋白酶和L-半胱氨酸混合液诱导骨关节炎模型(骨关节炎模型组)，左侧注射生理盐水(生理盐水对照组)。注射后第1，2，4，6周后分别取材，使用透射电镜观察股骨内侧髁关节软骨超微结构变化。 　　结果与结论：正常对照组和生理盐水对照组胞质内有丰富的粗面内质网、线粒体。骨关节炎模型组注射1周后，线粒体空泡化，可见轻度扩张的粗面内质网；2周后，出现脂滴，线粒体变性明显，空泡化严重，数目减少，粗面内质网扩张明显；4周后，脂滴增多，线粒体数目明显减少，大部分粗面内质网高度扩张，部分粗面内质网溶解断裂；6周后，胞质内见数个脂滴，大部分线粒体消失，仅有少量线粒体存在，大部分粗面内质网断裂溶解。说明木瓜蛋白酶诱导大鼠膝早期骨关节炎进程中透射电镜下软骨超微结构呈渐进性变化。
배경：목과단백매유도대서슬관절골성관절염시상용조모방법，능획득은정적골관절염모형。 　　목적：관찰목과단백매유도대서슬조기골관절염진정중투사전경하관절연골세포초미결구적변화규률。 　　방법：장18지SD대서수궤분위3조，2지위정상대조조불주간예；16지대서우슬관절강주사목과단백매화L-반광안산혼합액유도골관절염모형(골관절염모형조)，좌측주사생리염수(생리염수대조조)。주사후제1，2，4，6주후분별취재，사용투사전경관찰고골내측과관절연골초미결구변화。 　　결과여결론：정상대조조화생리염수대조조포질내유봉부적조면내질망、선립체。골관절염모형조주사1주후，선립체공포화，가견경도확장적조면내질망；2주후，출현지적，선립체변성명현，공포화엄중，수목감소，조면내질망확장명현；4주후，지적증다，선립체수목명현감소，대부분조면내질망고도확장，부분조면내질망용해단렬；6주후，포질내견수개지적，대부분선립체소실，부유소량선립체존재，대부분조면내질망단렬용해。설명목과단백매유도대서슬조기골관절염진정중투사전경하연골초미결구정점진성변화。
BACKGROUND:Papain-induced rat knee osteoarthritis is a common modeling method, which can obtain a stable osteoarthritis model. OBJECTIVE:To observe the change of ultrastructure of chondrocytes in the early process of papain-induced rat knee osteoarthritis under transmission electron microscope. METHODS:A total of 18 Sprague-Dawley rats were randomly divided into three groups. Two rats were considered as a normal control group, without intervention. The mixture of papain and L-cysteine was injected in right knee joint cavity of 16 rats to induce osteoarthritis models (osteoarthritis model group). Physiological saline was injected in the left side (physiological saline control group). At 1, 2, 4 and 6 weeks after injection, samples were col ected. Transmission electron microscope was used to observe the change of cartilage ultrastructure of the medial femoral condyle joint. RESULTS AND CONCLUSION:For the normal control group and physiological saline control group, their cytoplasm contained abundant rough endoplasmic reticulum and mitochondria. After 1 week of injection, mitochondria vacuoles and light expanded rough endoplasmic reticulum were visible. Two weeks later, lipid droplets appeared, mitochondria degeneration was distinct, vacuolization was serious and its number was reduced, and rough endoplasmic reticulum expansion was obvious. Four weeks later, lipid droplets became increased, and the number of mitochondria decreased significantly. Most of the rough endoplasmic reticula were highly expanded, and part of the rough endoplasmic reticula were dissolved and fractured. Six weeks later, a number of lipid droplets were visible in cytoplasm, most of the mitochondria disappeared, only a smal number of mitochondria existed, and most of the rough endoplasmic reticula were dissolved and fractured. These results confirmed that cartilage ultrastructure changes gradual y in the early process of papain-induced rat knee osteoarthritis under transmission electron microscope.