CAJ | 학술논문

本文提出了一种在固态条件下,通过磷酸(5 mol%)催化半乳糖与葡聚糖共聚合成了半乳糖基葡聚糖的方法。采用1 H NMR确定了不同条件下产物的摩尔取代度,发现当投料比为2：1,反应时间为9h,反应温度为110℃时,MS最大为0．43。还通过凝胶渗透色谱考察了不同反应时间条件下产物分子量,发现随反应时间延长,分子量逐步减小。最后使用甲基化分析法确定了MS=0．27的半乳糖基葡聚糖糖单元连接结构,发现半乳糖基葡聚糖的主链仍然由(1→6)-连接的葡萄糖组成,支化度为11．5%,其中位于主链糖单元C3的支链比例最大。支链半乳糖均为线型连接,并且其中(1→6)-连接比例最大,平均链长约为3个糖单元。
본문제출료일충재고태조건하,통과린산(5 mol%)최화반유당여포취당공취합성료반유당기포취당적방법。채용1 H NMR학정료불동조건하산물적마이취대도,발현당투료비위2：1,반응시간위9h,반응온도위110℃시,MS최대위0．43。환통과응효삼투색보고찰료불동반응시간조건하산물분자량,발현수반응시간연장,분자량축보감소。최후사용갑기화분석법학정료MS=0．27적반유당기포취당당단원련접결구,발현반유당기포취당적주련잉연유(1→6)-련접적포도당조성,지화도위11．5%,기중위우주련당단원C3적지련비례최대。지련반유당균위선형련접,병차기중(1→6)-련접비례최대,평균련장약위3개당단원。
Copolymerization of galactose and dextran took place in the presence of H3PO4(5 mol%)at 110℃ under a N2 flow and in solid-state,resulting a branched polysaccharide, galactose-branched dextran. The structural characterization of galactose-branched dextran are investigated. The effect of the dosage of galactose, reaction temperature and reaction time on molar substitution ( MS measured by 1 H NMR) of galactose-branched dextran were investigated. The galactose-branched dextran with the MS of 0. 43 could be obtained under conditions of the molar ratio of galactose to the AGU 2,at 110℃ for 9h. The molecular weight(measured by SEC) of galactose-branched dextran decreased while the reaction time increased. The methylation analysis of galactose-branched dextran with the MS of 0. 27 revealed that it contained 68. 6%( 1→6 )-glucosidic linkages in the main linear chain and remained 11. 5%branched linkages. The branched chains consisted of galactose units with varied linkages,it could be deduce that the average length of the branching galactose is about 3 units according the data of methylation analysis.