CAJ | 학술논문

目的：基于细胞模型以及高效液相色谱—质谱联用技术对通脉养心丸中心肌保护活性物质进行筛选研究。方法：利用高效液相制备技术对通脉养心丸进行制备分离，然后采用过氧化氢氧化损伤H9 c2大鼠心肌细胞模型对通脉养心丸活性组分进行筛选。利用高效液相色谱—质谱联用技术对活性组分进行分析，通过查阅文献和根据化合物裂解规律，鉴定推断出可能的活性化合物。结果：发现通脉养心丸中10个组分具有明显的心肌保护活性，考察了其中5个组分的半数有效浓度并发现其呈现良好的量效关系。从这5个组分中推断出11个可能的活性化合物，包含甘草酸、甘草香豆素、甘草利酮、Glyasperin A等。结论：筛选出通脉养心丸中心肌保护活性物质，有望为中药复方制剂活性物质筛选研究提供借鉴。
목적：기우세포모형이급고효액상색보—질보련용기술대통맥양심환중심기보호활성물질진행사선연구。방법：이용고효액상제비기술대통맥양심환진행제비분리，연후채용과양화경양화손상H9 c2대서심기세포모형대통맥양심환활성조분진행사선。이용고효액상색보—질보련용기술대활성조분진행분석，통과사열문헌화근거화합물렬해규률，감정추단출가능적활성화합물。결과：발현통맥양심환중10개조분구유명현적심기보호활성，고찰료기중5개조분적반수유효농도병발현기정현량호적량효관계。종저5개조분중추단출11개가능적활성화합물，포함감초산、감초향두소、감초리동、Glyasperin A등。결론：사선출통맥양심환중심기보호활성물질，유망위중약복방제제활성물질사선연구제공차감。
Objective: Based on cell model and HPLC-MS technology , to screen myocardial protection active compounds from traditional patent medicine Tongmai Yangxin pill ( TMYXP ) . Methods: Fractions of TMYXP were prepared by high performance liquid preparation technology . The cardioprotective effects of prepared fractions were tested on H2O2 oxidation-damaged H9c2 myocardiocytes.The active components were analyzed by high performance liquid chromatography ( HPLC) coupled with high resolution mass spectrometry .The possible active compounds were putatively identified by comparison of their MS ions and molecular weight with literatures . Results:Ten TMYXP components presented significant myocardial protective activities , 5 of which were investigated and presented good dose-effect relationships .Their median effective concentrations ( EC50 ) were respectively 11.66, 17.44, 13.10, 7.332, 15.15 μg/mL.Totally, 11 potential active compounds were analyzed and identified , including Glycyrrhizic acid , Glycycoumarin , Licoisoflavone , Ophiopogonin D′, Licoricon, Gancaonin L, Neoglycyrol, Emodin, Angeloylgomisin H, Angeloylgomisin Q and Glyasperin A .Conclusion:The myocardial protection active compounds of TMYXP were screened successfully .