CAJ | 학술논문

为获得可用于鸡传染性法氏囊病病毒（ IBDV）抗体检测的重组抗原 VP2蛋白，根据GenBank中发表的IBDV VP2序列设计一对特异性引物，应用RT－PCR技术克隆IBDV经典标准攻毒株（ BC6／85株）的VP2基因，插入质粒pET－32a中构建重组表达质粒pET－32a－VP2，经IPTG诱导后获得了以包涵体形式表达的重组蛋白。重组蛋白纯化后，Western－blot检测表明具有良好的反应原性。本研究为下步建立IBDV抗体的间接ELISA方法及新型疫苗的研制奠定了基础。
위획득가용우계전염성법씨낭병병독（ IBDV）항체검측적중조항원 VP2단백，근거GenBank중발표적IBDV VP2서렬설계일대특이성인물，응용RT－PCR기술극륭IBDV경전표준공독주（ BC6／85주）적VP2기인，삽입질립pET－32a중구건중조표체질립pET－32a－VP2，경IPTG유도후획득료이포함체형식표체적중조단백。중조단백순화후，Western－blot검측표명구유량호적반응원성。본연구위하보건립IBDV항체적간접ELISA방법급신형역묘적연제전정료기출。
In order to obtain recombinant VP2 antigen for chicken infectious bursal disease virus ( IBDV ) antibody detection, a pair of specific primers were designed according to the published sequence of IBDV VP2 gene. The VP2 gene was amplified by RT-PCR from IBDV BC6/85 strain and cloned into pET-32a vector, the recombinant plasmid pET-32a-VP2 was induced by IPTG to get the recombinant protein expressed in inclusion body forms. After the recombinant protein was purified, Western blot detection showed that the expressed protein had good antigenicity and could be used for IBDV antibody detection. The research provides a foundation for IBDV antibody detection research and development of new vaccines.