CAJ | 학술논문

目的：模拟和分析2例1型糖尿病（T1D）患者外周血TCR Vβ7的结构。方法以荧光定量PCR熔解曲线分析技术检测T1D患者外周血TCR Vβ7的谱系漂移，利用IMGT数据库和生物信息学软件（CPH Models 2.0 Server和 RasMol 2 Software）模拟分析其分子结构。结果2例T1D患者外周血的TCR Vβ7拥有极为相似的氨基酸序列“CASRTAGQYEQYFGPGTR”和“CASRTAGQYEQFFGPGTR”，唯一不同的是第十二个氨基酸残基，前者为酪氨酸（Y），后者为苯丙氨酸（F）。分子模拟显示，两者TCR Vβ7基因结构明显不同。结论不同T1D患者来源的TCR Vβ7即使具有相似或相同的氨基酸序列，分子结构未必相同；其可能来自于不同的T细胞克隆。
목적：모의화분석2례1형당뇨병（T1D）환자외주혈TCR Vβ7적결구。방법이형광정량PCR용해곡선분석기술검측T1D환자외주혈TCR Vβ7적보계표이，이용IMGT수거고화생물신식학연건（CPH Models 2.0 Server화 RasMol 2 Software）모의분석기분자결구。결과2례T1D환자외주혈적TCR Vβ7옹유겁위상사적안기산서렬“CASRTAGQYEQYFGPGTR”화“CASRTAGQYEQFFGPGTR”，유일불동적시제십이개안기산잔기，전자위락안산（Y），후자위분병안산（F）。분자모의현시，량자TCR Vβ7기인결구명현불동。결론불동T1D환자래원적TCR Vβ7즉사구유상사혹상동적안기산서렬，분자결구미필상동；기가능래자우불동적T세포극륭。
Objective To compare the sequences and crystal structures of TCR Vβ7 obtained from two patients with type 1 diabetes(T1D).Methods The skewness of TCR Vβ7 of two T1D patients were detected with real-time florescence quantitative polymerase chain reaction (RQ-PCR) and DNA melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to IMGT database, CPH Models2.0 Server and RasMol 2 Software.Results The whole sequences of TCR Vβ7 of T1D patient-1 were “CASRTAGQYEQYFGPGTR”, that of patient-2 were “CASRTAGQYEQFFGPGTR”, the only difference between them lied on the twelfth amino acid. The crystal structures of Vβ7 of the two patients simulated with stick model were rather similar, while that with sphere model were obviously different.Conclusion Although sharing the similar or same gene sequences, the crystal structures of the TCR Vβ7 obtained from different T1D patients probably are different.