中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2015年
7期
19-22
,共4页
方华%杨淼%张伟晶%章建平%张竞超%章放香
方華%楊淼%張偉晶%章建平%張競超%章放香
방화%양묘%장위정%장건평%장경초%장방향
瑞芬太尼聚己内酯%线粒体%体外循环
瑞芬太尼聚己內酯%線粒體%體外循環
서분태니취기내지%선립체%체외순배
Remifentanil polycaprolactone%Mitochondrion%Cardiopulmonary bypass
目的：观察犬体外循环（CPB）中REM-PCL对心肌能量代谢的影响。方法采用CPB心肌缺血再灌注模型，12只犬随机分为REM-PCL组（RP组，n=6）和对照组（C组，n=6）。RP组和C组分别于转机前静脉注射0.2mg/kg REM-PCL及等量生理盐水。分别于转机前、缺血60min和再灌注60min时，测定心肌线粒体肿胀度（MSD）、丙二醛（MDA）含量、腺苷酸（ATP、AMP、ADP、EC、TAN）、活性氧（ROS）和总抗氧化能力（T-AOC）。分别于转机前、再灌注30min和60min时测定平均动脉压（MAP）、心输出量（CO）和心率（HR）。结果两组与转机前比较，缺血后MDA、MSD和ROS均增加，TAN、EC、T-AOC及ATP含量均下降（P＜0.01）；C组再灌注后MDA、MSD和ROS均增加（P＜0.01），TAN、EC、T-AOC及ATP含量均下降（P＜0.01）；缺血60min和再灌注60min时RP组MDA、MSD和ROS均明显低于C组（P＜0.01），TAN、EC、T-AOC及ATP含量均明显高于C组（P＜0.01）。再灌注30min和60min时RP组MAP、CO和HR较C组恢复迅速（P＜0.01）。结论 REM-PCL通过保护心肌线粒体结构及改善缺血心肌能量代谢，减轻缺血心肌再灌注损伤。
目的：觀察犬體外循環（CPB）中REM-PCL對心肌能量代謝的影響。方法採用CPB心肌缺血再灌註模型，12隻犬隨機分為REM-PCL組（RP組，n=6）和對照組（C組，n=6）。RP組和C組分彆于轉機前靜脈註射0.2mg/kg REM-PCL及等量生理鹽水。分彆于轉機前、缺血60min和再灌註60min時，測定心肌線粒體腫脹度（MSD）、丙二醛（MDA）含量、腺苷痠（ATP、AMP、ADP、EC、TAN）、活性氧（ROS）和總抗氧化能力（T-AOC）。分彆于轉機前、再灌註30min和60min時測定平均動脈壓（MAP）、心輸齣量（CO）和心率（HR）。結果兩組與轉機前比較，缺血後MDA、MSD和ROS均增加，TAN、EC、T-AOC及ATP含量均下降（P＜0.01）；C組再灌註後MDA、MSD和ROS均增加（P＜0.01），TAN、EC、T-AOC及ATP含量均下降（P＜0.01）；缺血60min和再灌註60min時RP組MDA、MSD和ROS均明顯低于C組（P＜0.01），TAN、EC、T-AOC及ATP含量均明顯高于C組（P＜0.01）。再灌註30min和60min時RP組MAP、CO和HR較C組恢複迅速（P＜0.01）。結論 REM-PCL通過保護心肌線粒體結構及改善缺血心肌能量代謝，減輕缺血心肌再灌註損傷。
목적：관찰견체외순배（CPB）중REM-PCL대심기능량대사적영향。방법채용CPB심기결혈재관주모형，12지견수궤분위REM-PCL조（RP조，n=6）화대조조（C조，n=6）。RP조화C조분별우전궤전정맥주사0.2mg/kg REM-PCL급등량생리염수。분별우전궤전、결혈60min화재관주60min시，측정심기선립체종창도（MSD）、병이철（MDA）함량、선감산（ATP、AMP、ADP、EC、TAN）、활성양（ROS）화총항양화능력（T-AOC）。분별우전궤전、재관주30min화60min시측정평균동맥압（MAP）、심수출량（CO）화심솔（HR）。결과량조여전궤전비교，결혈후MDA、MSD화ROS균증가，TAN、EC、T-AOC급ATP함량균하강（P＜0.01）；C조재관주후MDA、MSD화ROS균증가（P＜0.01），TAN、EC、T-AOC급ATP함량균하강（P＜0.01）；결혈60min화재관주60min시RP조MDA、MSD화ROS균명현저우C조（P＜0.01），TAN、EC、T-AOC급ATP함량균명현고우C조（P＜0.01）。재관주30min화60min시RP조MAP、CO화HR교C조회복신속（P＜0.01）。결론 REM-PCL통과보호심기선립체결구급개선결혈심기능량대사，감경결혈심기재관주손상。
Objective To observe the effect of REM-PCL on myocardial energy metabolism in dogs during cardiopulmonary bypass.Methods Used CPB myocardial ischemia reperfusion model, 12 dogs were randomly divided into REM-PCL group (group RP,n=6) and control group (group C,n=6). RP group and C group respectively were given intravenous injection of 0.2mg/kg REM-PCL and normal saline before the driving of the rotary machine. Before the driving of the rotary machine, at 60 minutes of ischemia,and after 60 minutes reperfusion, myocardial mitochondrial swelling degree (MSD), malondialdehyde (MDA) content, adenylate (ATP, AMP, ADP, EC, TAN), reactive oxygen species (ROS) and total antioxidative capacity (T-AOC) were respectively determined. Before the driving of the rotary machine, at 60 minutes of ischemia,and after 60 minutes reperfusion, Mean arterial pressure (MAP), cardiac output (CO) and heart rate (HR) were measured. ResultsAfter ischemia, MDA, MSD and ROS of two groups were increased, and TAN, EC, T-AOC and ATP content were decreased(P<0.01). At 60 minutes of ischemia and reperfusion for 60 minutes, MDA, MSD and ROS of the RP group were significantly lower than those of C group (P<0.01), TAN, EC, T-AOC and ATP contents of the RP group were significantly higher than those of C group(P<0.01).At reperfusion for 30 minutes and 60 minutes, MAP, CO and HR of the RP group recovered more rapidly than those of group C (P<0.01).Conclusion Through the protection of myocardial mitochondrial structure and improve myocardial energy metabolism, REM-PCL can reduce myocardial ischemia reperfusion injury.
