医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2015年
10期
86-87,88
,共3页
胸腺五肽%衍生肽%杀菌活性
胸腺五肽%衍生肽%殺菌活性
흉선오태%연생태%살균활성
Thymopentin%derivedpeptides%bactericidal activity
目的：研究胸腺五肽及其衍生肽在体外对不同细菌的杀菌活性。方法：根据胸腺五肽结构分别设计合成4种衍生肽如下：①胸腺五肽P5；②直肽链P10；③直肽链P12；④环肽P环。采用琼脂铺板计数法，测定衍生肽的杀菌活性。结果：P5、P10、P12对G+菌和G-菌有一定杀菌活性，其杀菌活性由强到弱依次为P12>P10=P5，P环没有杀菌活性。结论：胸腺五肽及其衍生肽P5、P10、P12对G+菌和G-菌有较弱的杀菌活性，P12环经过修饰成环状后，无杀菌活性，这可能与其疏水性和分子结构有关系。
目的：研究胸腺五肽及其衍生肽在體外對不同細菌的殺菌活性。方法：根據胸腺五肽結構分彆設計閤成4種衍生肽如下：①胸腺五肽P5；②直肽鏈P10；③直肽鏈P12；④環肽P環。採用瓊脂鋪闆計數法，測定衍生肽的殺菌活性。結果：P5、P10、P12對G+菌和G-菌有一定殺菌活性，其殺菌活性由彊到弱依次為P12>P10=P5，P環沒有殺菌活性。結論：胸腺五肽及其衍生肽P5、P10、P12對G+菌和G-菌有較弱的殺菌活性，P12環經過脩飾成環狀後，無殺菌活性，這可能與其疏水性和分子結構有關繫。
목적：연구흉선오태급기연생태재체외대불동세균적살균활성。방법：근거흉선오태결구분별설계합성4충연생태여하：①흉선오태P5；②직태련P10；③직태련P12；④배태P배。채용경지포판계수법，측정연생태적살균활성。결과：P5、P10、P12대G+균화G-균유일정살균활성，기살균활성유강도약의차위P12>P10=P5，P배몰유살균활성。결론：흉선오태급기연생태P5、P10、P12대G+균화G-균유교약적살균활성，P12배경과수식성배상후，무살균활성，저가능여기소수성화분자결구유관계。
Objective To study the bactericidal activity of Thymopentin peptide and its derived peptides in vitro.Methods According to the amino acid sequence of Thymopentin,four peptides were synthesized respectively as fellow:①Thymopentin P5;②Straight peptide P10;③Straight peptideP12;④Ring peptide Pring,.The bactericidal activity was determined by agar plating-method.Results P5、P10、P12 possessed bactericidal activity against G+and G-bacteria,its bactericidal activity from strong to weak was P12>P10=P5. Pringhave no bactericidal activity.Conclusion Thymopentin peptide and its derived peptides P5、P10、P12 possess weak bactericidal activity against G+and G- bacteria .Pringhave no bactericidal activity.It’s bactericidal activity is related to hydrophobicity in the peptide molecule.
