中国药物与临床
中國藥物與臨床
중국약물여림상
CHINESE REMEDIES & CLINICS
2015年
5期
607-609
,共3页
郭婷婷%赵烨%史小荣%王晓璐
郭婷婷%趙燁%史小榮%王曉璐
곽정정%조엽%사소영%왕효로
大鼠%骨盆底%雌二醇%成纤维细胞
大鼠%骨盆底%雌二醇%成纖維細胞
대서%골분저%자이순%성섬유세포
Rats%Pelvic floor%Estradiol%Fibroblasts
目的：研究17β-雌二醇（17-βE2）对特定加力强度的雌性大鼠盆底肌成纤维细胞中基质金属蛋白酶-9（MMP-9）、金属蛋白酶组织抑制剂-1（TIMP-1）及β-联蛋白（β-catenin）的影响。方法原代培养雌性大鼠耻尾肌及周围筋膜并分离成纤维细胞，通过Flexercell 4000系统加载强度为10％、时间为12 h的机械应力，给予17-βE2干预，同时设置对照组（成纤维细胞组）以及机械应力组（强度为10％、时间为12 h的机械应力组）。荧光定量聚合酶链反应（PCR）测量各组MMP-9，TIMP-1及β-catenin mRNA的相对表达量。结果 RT-PCR结果显示，加力组与对照组相比，成纤维细胞中MMP-9 mRNA的相对表达量为2.690±0.020，高于对照组，差异有统计学意义（P＜0.05），TIMP-1 mRNA及β-catenin mRNA的相对表达量为0.370±0.010、0.430±0.010，低于对照组，差异有统计学意义（P＜0.05），17-βE2干预后，成纤维细胞中 MMP-9 mRNA相对表达量为1.523±0.068，高于对照组，低于加力组，差异有统计学意义（P＜0.05），TIMP-1 mRNA及β-catenin mRNA的相对表达量为0.600±0.010、0.630±0.010，低于对照组，高于加力组，差异有统计学意义（P＜0.05）。结论机械拉伸力可以使细胞形态和伸展方向发生改变，MMP-9表达增加，β-catenin及TIMP-1的表达下降，而17-βE2使得上述应力表现减弱。
目的：研究17β-雌二醇（17-βE2）對特定加力彊度的雌性大鼠盆底肌成纖維細胞中基質金屬蛋白酶-9（MMP-9）、金屬蛋白酶組織抑製劑-1（TIMP-1）及β-聯蛋白（β-catenin）的影響。方法原代培養雌性大鼠恥尾肌及週圍觔膜併分離成纖維細胞，通過Flexercell 4000繫統加載彊度為10％、時間為12 h的機械應力，給予17-βE2榦預，同時設置對照組（成纖維細胞組）以及機械應力組（彊度為10％、時間為12 h的機械應力組）。熒光定量聚閤酶鏈反應（PCR）測量各組MMP-9，TIMP-1及β-catenin mRNA的相對錶達量。結果 RT-PCR結果顯示，加力組與對照組相比，成纖維細胞中MMP-9 mRNA的相對錶達量為2.690±0.020，高于對照組，差異有統計學意義（P＜0.05），TIMP-1 mRNA及β-catenin mRNA的相對錶達量為0.370±0.010、0.430±0.010，低于對照組，差異有統計學意義（P＜0.05），17-βE2榦預後，成纖維細胞中 MMP-9 mRNA相對錶達量為1.523±0.068，高于對照組，低于加力組，差異有統計學意義（P＜0.05），TIMP-1 mRNA及β-catenin mRNA的相對錶達量為0.600±0.010、0.630±0.010，低于對照組，高于加力組，差異有統計學意義（P＜0.05）。結論機械拉伸力可以使細胞形態和伸展方嚮髮生改變，MMP-9錶達增加，β-catenin及TIMP-1的錶達下降，而17-βE2使得上述應力錶現減弱。
목적：연구17β-자이순（17-βE2）대특정가력강도적자성대서분저기성섬유세포중기질금속단백매-9（MMP-9）、금속단백매조직억제제-1（TIMP-1）급β-련단백（β-catenin）적영향。방법원대배양자성대서치미기급주위근막병분리성섬유세포，통과Flexercell 4000계통가재강도위10％、시간위12 h적궤계응력，급여17-βE2간예，동시설치대조조（성섬유세포조）이급궤계응력조（강도위10％、시간위12 h적궤계응력조）。형광정량취합매련반응（PCR）측량각조MMP-9，TIMP-1급β-catenin mRNA적상대표체량。결과 RT-PCR결과현시，가력조여대조조상비，성섬유세포중MMP-9 mRNA적상대표체량위2.690±0.020，고우대조조，차이유통계학의의（P＜0.05），TIMP-1 mRNA급β-catenin mRNA적상대표체량위0.370±0.010、0.430±0.010，저우대조조，차이유통계학의의（P＜0.05），17-βE2간예후，성섬유세포중 MMP-9 mRNA상대표체량위1.523±0.068，고우대조조，저우가력조，차이유통계학의의（P＜0.05），TIMP-1 mRNA급β-catenin mRNA적상대표체량위0.600±0.010、0.630±0.010，저우대조조，고우가력조，차이유통계학의의（P＜0.05）。결론궤계랍신력가이사세포형태화신전방향발생개변，MMP-9표체증가，β-catenin급TIMP-1적표체하강，이17-βE2사득상술응력표현감약。
Objective To investigate the effects of 17β-estradiol (17β-E2) on matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase (TIMP-1) and β-catenin in the specific stressed pelvic myofibroblast of female rats. Methods Primary culture of pubococcygeus and peripheral fascia cells of female rats were segregated into fi-broblast. Then, the fibroblast were loaded with 10%mechanical stress for 12 h by Flexercell 4000 tensile loading sys-tem, and intervened with 17β-E2. Meanwhile, the control group (the fibroblast group) and mechanical stress group (10% stress-loaded for 12 h) were set up. The relative expression levels of MMP-9, TIMP-1 and β-catenin mRNA in each group were measured by fluorescent quantitative polymerase chain reaction (PCR). Results RT-PCR showed that, compared with the control group, higher relative expression level of MMP-9 mRNA (2.690±0.020), and lower rel-ative expression levels of TIMP-1mRNA and β-catenin mRNA (0.370 ±0.010 and 0.430 ±0.010) in fibroblasts were found in the mechanical stress group, with statistically significant differences (P<0.05). After intervened with 17β-E2, the relative expression level of MMP-9 mRNA of fibroblast (1.523±0.068) was higher than that in the control group and lower than that in the mechanical stress group, with statistical difference ( P<0.05);whereas the relative expression levels of TIMP-1 mRNA and β-catenin mRNA (0.600±0.010 and 0.630±0.010, respectively) were lower than those in the control group and higher than those in the mechanical stress group, with statistical differences ( P<0.05). Conclu-sion Mechanical stress can change the morphology and stretching direction of fibroblasts, increase MMP-9 expres-sion, and decrease β-catenin and TIMP-1 expressions. These effects can be attenuated by 17β-estradiol.
