医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2015年
10期
46-47,48
,共3页
HPLC法%罗己降压片%盐酸吡哆辛%含量
HPLC法%囉己降壓片%鹽痠吡哆辛%含量
HPLC법%라기강압편%염산필치신%함량
HPLC%Luojijiangya tablets%Pyridoxine Hydrochloride%Content
目的：建立HPLC法测定罗己降压片中盐酸吡哆辛含量。方法：使用十八烷基硅烷键合硅胶为填充剂，以0.04%戊烷磺酸钠溶液（用冰醋酸调节p H至3.0）-甲醇(85：15)为流动相，检测波长为291n m进行测定。流速1.0m L?m i n-1，柱温35℃。结果：平均加样回收率盐酸吡哆辛为99.04%，RSD=0.17%(n=9)。盐酸吡哆辛在0.4000～1.600μg范围内，进样量与峰面积值呈良好的线性关系(r=0.9999)。结论：方法简便、结果准确、重现性好，可为罗己降压片的质量控制提供依据。
目的：建立HPLC法測定囉己降壓片中鹽痠吡哆辛含量。方法：使用十八烷基硅烷鍵閤硅膠為填充劑，以0.04%戊烷磺痠鈉溶液（用冰醋痠調節p H至3.0）-甲醇(85：15)為流動相，檢測波長為291n m進行測定。流速1.0m L?m i n-1，柱溫35℃。結果：平均加樣迴收率鹽痠吡哆辛為99.04%，RSD=0.17%(n=9)。鹽痠吡哆辛在0.4000～1.600μg範圍內，進樣量與峰麵積值呈良好的線性關繫(r=0.9999)。結論：方法簡便、結果準確、重現性好，可為囉己降壓片的質量控製提供依據。
목적：건립HPLC법측정라기강압편중염산필치신함량。방법：사용십팔완기규완건합규효위전충제，이0.04%무완광산납용액（용빙작산조절p H지3.0）-갑순(85：15)위류동상，검측파장위291n m진행측정。류속1.0m L?m i n-1，주온35℃。결과：평균가양회수솔염산필치신위99.04%，RSD=0.17%(n=9)。염산필치신재0.4000～1.600μg범위내，진양량여봉면적치정량호적선성관계(r=0.9999)。결론：방법간편、결과준학、중현성호，가위라기강압편적질량공제제공의거。
ObjectiveHPLC method was established for the determination of Pyridoxine Hydrochloride in Luojijiangya Tablets. Methods ODS C18 column was used. The mobile phase was 0.04% sodium pentanesulfonate(adjusted to pH 3.0 with acetic acid)-methanol(85:15). The detection wavelength was 291nm. The flow rate was 1.0 mL?min-1 with the column temperature at 35℃. Results The average recoveries were 99.04%,RSD=0.17%(n=9). The linear range of Pyridoxine was within 0.4000~1.600μg (r=0.9999). Conclusion T he method is simple, accurate, stableand reliable. It can be used for quality control of Luojijiangya tablets.
