CAJ | 학술논문

目的探讨锌指结构转录因子1(zinc finger E-box binding homeobox 1,ZEB1)和E钙黏蛋白在膀胱尿路上皮癌组织和正常膀胱黏膜组织中的表达情况、相关性及其与临床病理特征的关系.方法收集2008年3月至2011年3月86例膀胱尿路上皮癌患者的手术标本.男51例,女35例.年龄36 ～72岁,平均(64.1±10.2)岁.其中＞65岁40例,≤65岁46例.均经病理检查证实为膀胱尿路上皮癌,病理分级:G128例,G2～G358例.TNM分期:Ta～T1期34例,T2～T4期52例.肿瘤单发49例,多发37例.初发55例,复发31例.16例正常膀胱黏膜组织取自BPH患者开放手术标本.年龄51 ～75岁,平均(58.6±18.4)岁.采用免疫组化染色方法分别检测ZEB1和E钙黏蛋白在膀胱癌组织及正常膀胱黏膜组织中的表达情况,分析两者在膀胱癌组织中表达的相关性,以及两者表达情况与患者临床病理特征的关系.结果 ZEB1在膀胱癌组织和正常膀胱黏膜组织中表达的阳性率分别为39.5％(34/86)和0(0/16);在高分级(G2 ～ G3)和低分级(G1)膀胱癌组织中表达的阳性率分别为48.3％(28/58)和21.4％(6/28);在T2～T4期和Ta～T1期膀胱癌组织中表达的阳性率分别为51.9％ (27/52)和20.6％(7/34),差异均有统计学意义(P＜0.05).E钙黏蛋白在膀胱癌组织和正常膀胱黏膜组织中表达的异常率分别为61.6％ (53/86)和81.2％(13/16),差异有统计学意义(P＜0.01);在高分级(G2 ～ G3)和低分级(G1)膀胱癌组织中表达的异常率分别为53.4％(31/58)和78.6％(22/28);在T2～ T4期和Ta～T1期膀胱癌组织中表达的异常率分别为51.9％(27/52)和76.5％(26/34),差异均有统计学意义(P＜0.05).在膀胱癌组织中,ZEB1的阳性表达与E钙黏蛋白的异常表达间呈负相关(r =0.247,P＜0.05).结论 ZEB1和E钙黏蛋白在膀胱癌组织中的表达具有负相关性,且两者的表达与膀胱癌的分期、分级密切相关.
목적 탐토자지결구전록인자1(zinc finger E-box binding homeobox 1,ZEB1)화E개점단백재방광뇨로상피암조직화정상방광점막조직중적표체정황、상관성급기여림상병리특정적관계.방법 수집2008년3월지2011년3월86례방광뇨로상피암환자적수술표본.남51례,녀35례.년령36 ～72세,평균(64.1±10.2)세.기중＞65세40례,≤65세46례.균경병리검사증실위방광뇨로상피암,병리분급:G128례,G2～G358례.TNM분기:Ta～T1기34례,T2～T4기52례.종류단발49례,다발37례.초발55례,복발31례.16례정상방광점막조직취자BPH환자개방수술표본.년령51 ～75세,평균(58.6±18.4)세.채용면역조화염색방법분별검측ZEB1화E개점단백재방광암조직급정상방광점막조직중적표체정황,분석량자재방광암조직중표체적상관성,이급량자표체정황여환자림상병리특정적관계.결과 ZEB1재방광암조직화정상방광점막조직중표체적양성솔분별위39.5％(34/86)화0(0/16);재고분급(G2 ～ G3)화저분급(G1)방광암조직중표체적양성솔분별위48.3％(28/58)화21.4％(6/28);재T2～T4기화Ta～T1기방광암조직중표체적양성솔분별위51.9％ (27/52)화20.6％(7/34),차이균유통계학의의(P＜0.05).E개점단백재방광암조직화정상방광점막조직중표체적이상솔분별위61.6％ (53/86)화81.2％(13/16),차이유통계학의의(P＜0.01);재고분급(G2 ～ G3)화저분급(G1)방광암조직중표체적이상솔분별위53.4％(31/58)화78.6％(22/28);재T2～ T4기화Ta～T1기방광암조직중표체적이상솔분별위51.9％(27/52)화76.5％(26/34),차이균유통계학의의(P＜0.05).재방광암조직중,ZEB1적양성표체여E개점단백적이상표체간정부상관(r =0.247,P＜0.05).결론 ZEB1화E개점단백재방광암조직중적표체구유부상관성,차량자적표체여방광암적분기、분급밀절상관.
Objective To explore the expression,correlation and clinic-pathological characters of zinc finger E-box binding homeobox 1 (ZEB1) and E-cadherin in normal bladder tissues and bladder urothelial carcinoma(BUC).Methods Immunohistochemical staining was performed on paraffin embedded sections to detect the expression of ZEB1 and E-cadherin in 86 cases of BUC,diagnosed by pathology,between March 2008 and March 2011.Among them,51 cases were male and 35 cases were female.The mean age was (64.1 ± 10.2)years,range 36 to 72 years.40 cases were older than 65 years old,and 46 cases were less than 65 years old.The pathological classification included G1 in 28 cases,G2-G3 in 58 cases.The TNM classification showed that Ta-T1 stage in 34 cases,T2-T4 in 52 cases.Single lesion was found in 49 cases and multi lesion was found in 37 cases.55 cases were primary lesion;the other 31 cases were diagnosed as tumor recurrence.Normal bladder mucosa was obtained from 16 patients with BPH.Their age ranged from 51 to 75 years old,mean (58.6 ± 18.4) years old.Similar immunohistochemical staining about ZEB1 and E-cadherin was performed.The expression of ZEB1 and E-cadherin in different tissue and its relationship with the clinic pathological were further analyzed.Results The positive rate of ZEB1 in bladder cancer group (39.5％,34/86) was significantly higher than that in normal bladder mucosa group (0,0/16) (P ＜ 0.01).The positive rate of ZEB1 in high grade bladder cancer (G2-G3) was 48.3％ (28/58),which was significant higher than that in low grade bladder cancer (G1) (21.4％,6/28) (P ＜0.05);The positive rate of ZEB1 in muscle-invasive bladder cancer (T2-T4) was 51.9％ (27/52),which was significant higher than that in non-muscle-invasive cancer (Ta-T1) (20.6％,7/34) (P ＜ 0.01).The abnormal rate of E-cadherin expression in bladder cancer (61.6％,53/86) was significantly lower than that in normal bladder mueosa (81.2％,13/16) (P ＜ 0.01).The abnormal expression rate of E-cadherin in high grade bladder cancer (G2-G3) (53.4％,31/58) was significantly lower than that in low grade bladder cancer (G1) (78.6％,22/28)(P ＜ 0.05);The abnormal rate of E-cadherin expression in invasive bladder cancer (T2-T,4) was 51.9％ (27/52),which was lower than that in non-muscle invasive (Ta-T1) cancer (76.5％,26/34) (P ＜ 0.05).In bladder cancer,there was a negative correlation between the abnormal expression of E-cadherin and positive expression of ZEB1 (r =0.247,P ＜ 0.05).Conclusion The expressions of ZEB1 and E-cadherin in bladder cancer were negatively correlated,which had high correlated with tumor grade and stage.