CAJ | 학술논문

目的：探讨白细胞介素‐1受体拮抗剂基因（IL‐1RN ）第2号外显子＋8006T／C位点基因多态性在慢性乙型肝炎患者中的分布及其相关性。方法采用聚合酶链反应‐限制性片段长度多态性的分析方法，对450例慢性乙型肝炎患者和420例对照者IL‐1RN（T＋8006C）位点基因多态性进行检测，计算并分析其等位基因和基因型频率。结果观察组和对照组IL‐1RN（T＋8006C）位点基因型的分布最常见为TT型，TC型次之，CC型则较为罕见；在不同年龄组、不同性别间其分布比较，差异无统计学意义（P＞0．05）；观察组C型等位基因及 TC基因型频率明显低于正常对照组，差异有统计学意义（P＜0．01）。结论 IL‐1RN（T＋8006C）位点等位基因及基因型的分布不存在年龄和性别间的区别，但与慢性乙型肝炎存在相关性，在乙型肝炎病毒感染中，C型等位基因可能具有保护作用。
목적：탐토백세포개소‐1수체길항제기인（IL‐1RN ）제2호외현자＋8006T／C위점기인다태성재만성을형간염환자중적분포급기상관성。방법채용취합매련반응‐한제성편단장도다태성적분석방법，대450례만성을형간염환자화420례대조자IL‐1RN（T＋8006C）위점기인다태성진행검측，계산병분석기등위기인화기인형빈솔。결과관찰조화대조조IL‐1RN（T＋8006C）위점기인형적분포최상견위TT형，TC형차지，CC형칙교위한견；재불동년령조、불동성별간기분포비교，차이무통계학의의（P＞0．05）；관찰조C형등위기인급 TC기인형빈솔명현저우정상대조조，차이유통계학의의（P＜0．01）。결론 IL‐1RN（T＋8006C）위점등위기인급기인형적분포불존재년령화성별간적구별，단여만성을형간염존재상관성，재을형간염병독감염중，C형등위기인가능구유보호작용。
Objective To investigate the distribution and correlation of gene polymorphism in the second exon of interleukin‐1 receptor antagonist gene at position +8006[IL‐1RN(T+8006C)] in patients with chronic hepatitis B .Methods The IL‐1RN(T+8006C) polymorphism was detected by polymerase chain reaction and restriction frag‐ment length polymorphisms methods in 450 patients with chronic hepatitis B (observation group) and 420 healthy persons(control group) ,then the genotype and allele frequencies were calculated and analyzed .Results In the obser‐vation group and control group ,the TT genotype was the most frequent ,then the TC genotype ,and CC genotype was rare .The distribution of genotypes and allele was no different among different gender and age groups (P>0 .05) .The frequencies of TC genotype and C allele were lower in patients with chronic hepatitis B than that of the control group (P<0 .01) .Conclusion The distribution of IL‐1RN(T+8006C) genotypes and allele were no difference among dif‐ferent gender and age groups ,but it has correlation with chronic hepatitis B .Allele C might be a protect factor against hepatitis B virus infection .