微循环学杂志
微循環學雜誌
미순배학잡지
CHINESE JOURNAL OF MICROCIRCULATION
2015年
2期
22-26
,共5页
人血小板裂解液%间充质干细胞%羊膜来源%成骨分化
人血小闆裂解液%間充質榦細胞%羊膜來源%成骨分化
인혈소판렬해액%간충질간세포%양막래원%성골분화
Human platelet lysate%Mesenchymal stem cells%Amnion%Osteogenic differentiation
目的：：研究人血小板裂解液(HPL)对羊膜来源间充质干细胞(AMMSCs)成骨分化的作用。方法：分别以含7% HPL(HPL组)和10%胎牛血清(FBS,FBS 组)的 LG-DMEM培养介质扩增 AMMSCs,比较两组扩增AMMSCs效率及其免疫表型 SSEA-3和 SSEA-4的表达差异。使用 MSCs 成骨分化培养液诱导 AMMSCs 成骨,对比观察两组钙盐沉积量、钙化结节、碱性磷酸酶(ALP)活性；提取两组成骨诱导后 AMMSCs 总 RNA,采用 RT-PCR检测成骨分化调节因子RUNX-2和ALP mRNA相对表达量。结果：HPL组扩增速度快于FBS组；AMMSCs的 SSEA-3和 SSEA-4表达较 FBS 组明显下调(P<0.05)。HPL 组钙盐沉积量、钙化结节数量、ALP 活性以及RUNX-2和 ALP mRNA表达量均明显高于 FBS组(P均<0.05)。结论：含 HPL培养介质可促进 AMMSCs 成骨分化。
目的：：研究人血小闆裂解液(HPL)對羊膜來源間充質榦細胞(AMMSCs)成骨分化的作用。方法：分彆以含7% HPL(HPL組)和10%胎牛血清(FBS,FBS 組)的 LG-DMEM培養介質擴增 AMMSCs,比較兩組擴增AMMSCs效率及其免疫錶型 SSEA-3和 SSEA-4的錶達差異。使用 MSCs 成骨分化培養液誘導 AMMSCs 成骨,對比觀察兩組鈣鹽沉積量、鈣化結節、堿性燐痠酶(ALP)活性；提取兩組成骨誘導後 AMMSCs 總 RNA,採用 RT-PCR檢測成骨分化調節因子RUNX-2和ALP mRNA相對錶達量。結果：HPL組擴增速度快于FBS組；AMMSCs的 SSEA-3和 SSEA-4錶達較 FBS 組明顯下調(P<0.05)。HPL 組鈣鹽沉積量、鈣化結節數量、ALP 活性以及RUNX-2和 ALP mRNA錶達量均明顯高于 FBS組(P均<0.05)。結論：含 HPL培養介質可促進 AMMSCs 成骨分化。
목적：：연구인혈소판렬해액(HPL)대양막래원간충질간세포(AMMSCs)성골분화적작용。방법：분별이함7% HPL(HPL조)화10%태우혈청(FBS,FBS 조)적 LG-DMEM배양개질확증 AMMSCs,비교량조확증AMMSCs효솔급기면역표형 SSEA-3화 SSEA-4적표체차이。사용 MSCs 성골분화배양액유도 AMMSCs 성골,대비관찰량조개염침적량、개화결절、감성린산매(ALP)활성；제취량조성골유도후 AMMSCs 총 RNA,채용 RT-PCR검측성골분화조절인자RUNX-2화ALP mRNA상대표체량。결과：HPL조확증속도쾌우FBS조；AMMSCs적 SSEA-3화 SSEA-4표체교 FBS 조명현하조(P<0.05)。HPL 조개염침적량、개화결절수량、ALP 활성이급RUNX-2화 ALP mRNA표체량균명현고우 FBS조(P균<0.05)。결론：함 HPL배양개질가촉진 AMMSCs 성골분화。
Objective:To investigate the effect of human platelet lysate (HPL)on amnion-derived mesenchy-mal stem cells (AMMSCs)osteogenic differentiation.Method:AMMSCs were amplified by LG-DMEM medium supplemented with 7% HPL(HPL group)or 10% fetal bovine serum (FBS,FBS group).The surface molecular markers potential SSEA-3 and SSEA-4 were compared.When AMMSCs were used for osteogenic differentiation, ALP activity and calcium deposition were comparatively observed.Total RNA was isolated and the gene expression of RUNX-2 and ALP were investigated by RT-PCR.Results:HPL-containing medium significantly accelerated MSCs proliferation as compared with FBS containing medium.Expressions of embryonic stem cell markers SSEA-3 and SSEA-4 on AMMSCs were promoted down-regulation by HPL-containing medium than that of FBS-containing medium.HPL-containing medium significantly improved ALP activity and calcium deposition,which also enhanced the mRNA level of RUNX-2 and ALP (P<0.05).Conclusion:HPL-containing medium promotes osteogenic differ-entiation of AMMSCs.
