CAJ | 학술논문

目的：：观察罗布麻叶提取物(AVLE)预处理对心肌缺血再灌注(MI/R)大鼠心肌细胞凋亡和细胞信号转导系统中丝(苏)氨酸激酶(Akt)和细胞外信号调节激酶(ERK1/2)的影响。方法：采用 SD大鼠 MI/R模型(结扎冠脉左前降支起始部30min 后再灌注4h),随机分为 Sham 组(假手术)、MI/R 组、AVLE 预处理组[AVLE (500mg/kg)灌胃,每日一次,连续灌胃7天后再行 MI/R]。用 TUNEL 法检测各组心肌细胞凋亡,计算凋亡指数(AI)；荧光免疫分析法检测各组心肌组织凋亡蛋白Caspase-3活性；Western Blotting 测定心肌组织 Akt和 ERK1/2的表达水平和磷酸化水平。结果：与 Sham组比较,MI/R组心肌细胞 AI显著升高(P<0.05),心肌组织Caspase-3活性明显升高(P<0.05),心肌 Akt和 ERK1/2的磷酸化水平显著降低(P<0.05)；与 MI/R组比较,AVLE预处理组 AI明显下降(P<0.05),心肌组织Caspase-3活性显著降低(P<0.05),Akt和 ERK1/2磷酸化水平显著升高(P<0.05)。结论：AVLE 能够抑制缺血再灌注所致心肌细胞损伤,其作用与生存信号通路蛋白 PI3K/Akt 和ERK1/2的活化水平有关。
목적：：관찰라포마협제취물(AVLE)예처리대심기결혈재관주(MI/R)대서심기세포조망화세포신호전도계통중사(소)안산격매(Akt)화세포외신호조절격매(ERK1/2)적영향。방법：채용 SD대서 MI/R모형(결찰관맥좌전강지기시부30min 후재관주4h),수궤분위 Sham 조(가수술)、MI/R 조、AVLE 예처리조[AVLE (500mg/kg)관위,매일일차,련속관위7천후재행 MI/R]。용 TUNEL 법검측각조심기세포조망,계산조망지수(AI)；형광면역분석법검측각조심기조직조망단백Caspase-3활성；Western Blotting 측정심기조직 Akt화 ERK1/2적표체수평화린산화수평。결과：여 Sham조비교,MI/R조심기세포 AI현저승고(P<0.05),심기조직Caspase-3활성명현승고(P<0.05),심기 Akt화 ERK1/2적린산화수평현저강저(P<0.05)；여 MI/R조비교,AVLE예처리조 AI명현하강(P<0.05),심기조직Caspase-3활성현저강저(P<0.05),Akt화 ERK1/2린산화수평현저승고(P<0.05)。결론：AVLE 능구억제결혈재관주소치심기세포손상,기작용여생존신호통로단백 PI3K/Akt 화ERK1/2적활화수평유관。
Objective:To investigate the effect of apocynum venetum leaf extract (AVLE)on myocardium is-chemia reperfusion(MI/R)inj ury rat and signal pathway serine/threonine kinase (Akt)and the mitogen-activated protein kinase p42/44extra-cellular signa-l regulated kinases (ERK1/2).Method:Male Sprague-Dawley rats were divided into 3 groups randomly:sham,ischemia reperfusion,AVLE preconditioned[(500mg/kg/d,o.g.)once dai-ly for one week]+MI/R.The model of myocardial I/R inj ury in vivo was made by ligating the left anterior descend-ing artery for 30 min followed by 4 h of reperfusion in SD rats.Cardiomyocyte apoptosis was detected using in situ TDT-mediated dUTP nick end labeling (TUNEL).The activity of Caspase-3 was determined by fluorescent assay. The expressions and phosphorylations of Akt and ERK1/2 were measured using Western blot.Results:Compared with Sham group,the apoptosis index and the expressions of Caspase-3 were significantly increased and the phos-phorylations of both Akt and ERK1/2 in I/R cardiac issue were significantly attenuated in MI/R group (P<0.05). Compared with I/R group,the apoptosis index and the expressions of Caspase-3 were significantly attenuated in AVLEpreconditioned animals (P<0.05).AVLE preconditioning significantly increased the phosphorylations of both Akt and ERK1/2 in I/R cardiac issue (P<0.05).Conclusion:The results suggest that AVLE preconditioned may be a-ble to protect the heart against reperfusion-induced inj ury mediated by the increase of phosphorylating Akt and ERK1/2.