CAJ | 학술논문

针对单一植物源杀菌剂存在高成本、低抗菌性且抗菌谱较窄及植物精油不稳定的问题，设计开发了一种新型的抗菌复方精油微乳液。采用协同指数法确定精油的最佳复配组合，并利用相转化法制备其微乳液，通过分析拟三元相图中微乳液区域的大小研究了表面活性剂、助表面活性剂、表面活性剂与助表面活性剂的质量比(Km)及油相含量对微乳液形成的影响。结果表明：在室温条件下，以丁香油-肉桂油-大蒜油复配精油(质量比8∶1∶8)为油相、Tween-80为表面活性剂、甘油为助表面活性剂、蒸馏水为水相，当 Km＝5∶1、油相和混合表面活性剂的质量比为2∶8时，制备的微乳液抗菌性能显著，且外观及稳定性等均较好。
침대단일식물원살균제존재고성본、저항균성차항균보교착급식물정유불은정적문제，설계개발료일충신형적항균복방정유미유액。채용협동지수법학정정유적최가복배조합，병이용상전화법제비기미유액，통과분석의삼원상도중미유액구역적대소연구료표면활성제、조표면활성제、표면활성제여조표면활성제적질량비(Km)급유상함량대미유액형성적영향。결과표명：재실온조건하，이정향유-육계유-대산유복배정유(질량비8∶1∶8)위유상、Tween-80위표면활성제、감유위조표면활성제、증류수위수상，당 Km＝5∶1、유상화혼합표면활성제적질량비위2∶8시，제비적미유액항균성능현저，차외관급은정성등균교호。
In order to overcome the high cost,low antimicrobial activity,and narrow antibacterial spectrum of sin-gle botanical fungicide as well as instability of plant essential oils,a new antimicrobial micro-emulsion containing compound essential oils was designed and developed. The optimal compound ratio of essential oils was determined using the synergy index method,and its micro-emulsion was prepared by phase inversion method. The influencesof surfactant,co-surfactant,mass ratio(Km) of surfactant to co-surfactant,and content of oil phase on the micro-emulsion formation were investigated through analyzing the micro-emulsion area of pseudo ternary phase diagram. The results show that the oil phase was composed mainly of clove oil,cinnamon oil and garlic oil with the mass ratio of 8∶1∶8,and the water phase was distilled water. The surfactant and co-surfactant were Tween-80 and glycerol, respectively. WhenKm was 5∶1 and the mass ratio of oil phase to mixed surfactants was 2∶8,the antibacterial micro-emulsion could achieve the maximum activity as well as exhibit a good appearance and stability at room temperature.