CAJ | 학술논문

氰化物(cyanide)对生物有机体和环境都有剧毒。在非生物胁迫条件下，植物细胞内会产生大量的氰化物，这些氰化物对细胞具有毒害作用，基因β-CAS 和 NIT 是玉米细胞氰化物解毒途径上的重要基因。本研究分别以200,mmol/L NaCl胁迫条件下第2,h、8,h、24,h和48,h的天塔5号(F1)及其亲本三叶期的幼苗作为实验材料，对其光合速率、生长势、叶片氰化物含量及氰化物解毒途径重要相关基因β-CAS和NIT的表达量进行了检测和统计学分析。研究结果表明，F1代的根长显著长于亲本；在盐胁迫处理下，三系叶片的光合速率均发生下调，其中 F1的光合速率和母本没有显著差异，但是显著高于父本；F1叶片的氰化物含量比母本和父本更早发生下降；三系的基因β-CAS 表达量均上调，上调幅度从大到小的顺序依次是母本、F1和父本；F1和母本的 NIT 基因表达量下调，父本则发生上调。
청화물(cyanide)대생물유궤체화배경도유극독。재비생물협박조건하，식물세포내회산생대량적청화물，저사청화물대세포구유독해작용，기인β-CAS 화 NIT 시옥미세포청화물해독도경상적중요기인。본연구분별이200,mmol/L NaCl협박조건하제2,h、8,h、24,h화48,h적천탑5호(F1)급기친본삼협기적유묘작위실험재료，대기광합속솔、생장세、협편청화물함량급청화물해독도경중요상관기인β-CAS화NIT적표체량진행료검측화통계학분석。연구결과표명，F1대적근장현저장우친본；재염협박처리하，삼계협편적광합속솔균발생하조，기중 F1적광합속솔화모본몰유현저차이，단시현저고우부본；F1협편적청화물함량비모본화부본경조발생하강；삼계적기인β-CAS 표체량균상조，상조폭도종대도소적순서의차시모본、F1화부본；F1화모본적 NIT 기인표체량하조，부본칙발생상조。
Cyanide is poisonous to live organisms and environment. The cyanide content would burst out in cells when the plants are under the antibiotic stress,which is harmful to plants. Genesβ-CAS andNIT are important genes involved in the cyanide detoxication. The Tianta 5(F1),and its parent lines were treated with 200 mmol/L NaCl for 2 h,8 h,24 h and 48 h,and the same lines were used as the control group when they were at three-leaf stage. The photosynthetic rate,the plant character,the cyanide content and the gene expression ofβ-CAS andNIT of Tianta 5 and its parent lines were detected. Then the data were analyzed statistically. The results indicated that the root of F1 was significantly longer than those of the parent lines;the photosynthetic rates of all lines were down-regulated after treated by salt,among which no significant difference was found between F1 and female parent.However,the pho-tosynthetic rate of the male parent is significantly lower than that of F1.The cyanide content of F1 decreased earlier than its parents and the expression level ofβ-CAS was up-regulated for all lines,the female parent increasingthe most while the male parent the least.The expression level ofNIT of F1 and female parent was down-regulated while that of the male parent was up-regulated.