CAJ | 학술논문

侵袭性真菌感染在近些年的发病率和病死率显著上升,对人类尤其是免疫功能低下患者的健康构成了重大威胁.能够尽早确诊、绘制侵袭性真菌感染病原谱是提高侵袭性真菌感染治愈率、降低病死率的关键.分子生物学技术的进步为侵袭性真菌感染的诊断提供了新的思路,与传统方法相比,分子诊断技术大大缩短了诊断所需时间,同时提高了敏感性和特异性,且操作简便、易于重复.本文对研究较多的分子诊断技术如聚合酶链反应技术、基因测序等以及侵袭性真菌感染病原谱调查进行综述.
침습성진균감염재근사년적발병솔화병사솔현저상승,대인류우기시면역공능저하환자적건강구성료중대위협.능구진조학진、회제침습성진균감염병원보시제고침습성진균감염치유솔、강저병사솔적관건.분자생물학기술적진보위침습성진균감염적진단제공료신적사로,여전통방법상비,분자진단기술대대축단료진단소수시간,동시제고료민감성화특이성,차조작간편、역우중복.본문대연구교다적분자진단기술여취합매련반응기술、기인측서등이급침습성진균감염병원보조사진행종술.
In recent years,morbidity and mortality of invasive fungal infections (IFI) has increased significantly,especially in immunodeficiency patients.Early diagnosis and making IFIpathogenic spectrum are the keys to guide the anti-fungal treatment and reduce the mortality.Advances in molecular biology techniques provide a new way for the diagnosis of IFI.Compared with the traditional technology,molecular diagnostic technology is rapid,high-sensitive and specific,and easy to operate and repeat.This paper reviews advancements on IFI molecular diagnostic technologies such as polymerase chain reaction,gene sequencing and IFI pathogen spectrum.