中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2015年
5期
491-495
,共5页
边富宁%吴媛%于栓宝%车洁%李文革%邵祝军%朱兵清%卢金星
邊富寧%吳媛%于栓寶%車潔%李文革%邵祝軍%硃兵清%盧金星
변부저%오원%우전보%차길%리문혁%소축군%주병청%로금성
新生隐球菌%格特隐球菌%基因型%交配型%毒力特性
新生隱毬菌%格特隱毬菌%基因型%交配型%毒力特性
신생은구균%격특은구균%기인형%교배형%독력특성
Cryptococcus neoformans%Cryptococcus gattii%Genotype%Mating type%Characteristics of virulence
目的 研究广西贵港地区新生隐球菌和格特隐球菌临床分离株,在种、基因型和交配型的构成及毒力特性.方法 收集2009-2012年广西贵港地区临床分离的新生隐球菌株20株,PCR扩增转录间隔区(ITS)序列进行分子生物学验证,通过特异性引物进行PCR扩增,鉴定血清型、交配型.利用M13-PCR指纹图谱法和URA5-限制性片段长度多态性分析法,鉴定和验证基因型.表型研究包括37 ℃生长试验、黑色素生成试验和尿素酶试验.结果 20株有19株为新生隐球菌格鲁比变种,血清型为A型,α交配型,基因型为VNⅠ型.1株为格特隐球菌,属α交配型,基因型VG Ⅰ.毒力特性研究结果显示,所有临床分离株37℃正常生长,黑色素试验、尿素酶试验阳性.结论 广西贵港地区隐球菌致病菌株中绝大多数(95％)为新生隐球菌格鲁比变种、血清型为A型,α交配型,基因型为VNⅠ型,并发现格特隐球菌菌株.
目的 研究廣西貴港地區新生隱毬菌和格特隱毬菌臨床分離株,在種、基因型和交配型的構成及毒力特性.方法 收集2009-2012年廣西貴港地區臨床分離的新生隱毬菌株20株,PCR擴增轉錄間隔區(ITS)序列進行分子生物學驗證,通過特異性引物進行PCR擴增,鑒定血清型、交配型.利用M13-PCR指紋圖譜法和URA5-限製性片段長度多態性分析法,鑒定和驗證基因型.錶型研究包括37 ℃生長試驗、黑色素生成試驗和尿素酶試驗.結果 20株有19株為新生隱毬菌格魯比變種,血清型為A型,α交配型,基因型為VNⅠ型.1株為格特隱毬菌,屬α交配型,基因型VG Ⅰ.毒力特性研究結果顯示,所有臨床分離株37℃正常生長,黑色素試驗、尿素酶試驗暘性.結論 廣西貴港地區隱毬菌緻病菌株中絕大多數(95％)為新生隱毬菌格魯比變種、血清型為A型,α交配型,基因型為VNⅠ型,併髮現格特隱毬菌菌株.
목적 연구엄서귀항지구신생은구균화격특은구균림상분리주,재충、기인형화교배형적구성급독력특성.방법 수집2009-2012년엄서귀항지구림상분리적신생은구균주20주,PCR확증전록간격구(ITS)서렬진행분자생물학험증,통과특이성인물진행PCR확증,감정혈청형、교배형.이용M13-PCR지문도보법화URA5-한제성편단장도다태성분석법,감정화험증기인형.표형연구포괄37 ℃생장시험、흑색소생성시험화뇨소매시험.결과 20주유19주위신생은구균격로비변충,혈청형위A형,α교배형,기인형위VNⅠ형.1주위격특은구균,속α교배형,기인형VG Ⅰ.독력특성연구결과현시,소유림상분리주37℃정상생장,흑색소시험、뇨소매시험양성.결론 엄서귀항지구은구균치병균주중절대다수(95％)위신생은구균격로비변충、혈청형위A형,α교배형,기인형위VNⅠ형,병발현격특은구균균주.
Objective To understand the species,genotypes and mating types of Cryptococcus neoformans and Cryptococcus gattii isolated from clinical samples in Guigang,Guangxi Zhuang Autonomous Region.Methods A total of 20 Cryptococcus strains were isolated from clinical samples in Guigang from 2009 to 2012.The biological identification was conducted by polymerase chain reaction (PCR) to amplify internal transcribed spacer (ITS) sequences.The serotypes and mating types of C.neoformans and C.gattii were identified by PCR with serotype-specific and mating type-specific primers.The genotype was characterized by PCR fingerprinting and URA5 gene restriction fragment length polymorphism (URA5-RFLP).Phenotype study included growth test at 37 ℃,melanin production test and urease test.Results Among the 20 strains,19 (95％) were identified as C.neoformans varieties (var.) grubii (serotype A,mating type α,genotype VN Ⅰ),and only 1 was identified as C.gattii (mating type α,genotype VG Ⅰ).The results of virulence test showed that all the strains grew well at 37 ℃ and positive in both urease test and melanin production test.Conclusion C.neoformans var.grubii (serotype A,genotype VN Ⅰ and mating type αt) was the predominant pathogen causing cryptococcosis in Guigang,and C.gattii strain was also detected.
